酶法制备复合氨基酸钙工艺的研究

Study on Synthesis Amino Acid Calcium by Technology of Fractional Hydrolyze

初步研究了酶法分步水解鸡块、鸡骨来制备复合氨基酸钙的工艺,讨论了酶水解的最佳条件以及复合氨基酸溶液与游离钙的最佳螯合工艺条件,确定酶水解的最佳工艺条件为:第一步添加胰蛋白酶200u/g,pH值为7,酶解温度为50℃,固液比为1∶6,时间为2h;第二步添加木瓜蛋白酶1200u/g,pH值为7.0,酶解温度为70℃,固液比为1∶6,时间为4h,水解度可达32.29%,钙溶率为25.3%。螯合最佳工艺条件为:pH值为8.0,酶解温度为70℃,配体摩尔比(AA/Ca)为1∶2。

This paper preliminary studies the synthesis of compound amino acid calcium by the technology of fractional hydrolyze on the chicken bones, and discusses the best hydrolytic factors and the best chelating factors between compound amino acid and calcium. The best hydrolytic factors are： the first step the enzyme concentration of pancreatin 200 u/g, pH 7.0, temperature 50℃, the solid and the liquid ratio 1 to 6, time 2 hours; the second step pawpaw albumen-enzyme 1 200 u/g, pH 7.0, temperature 70℃, the solid and the liquid ratio 1 to 6, time 4 hours. Studies showed that the hydrolysis rate was 32.29% and the solubility rate of calcium was 25.3%. The best chelating factors were pH 8.0, temperature 70 ℃, the molar ratio of ligand （amino acid and calcium） 2 ： 1.