摘要
目的通过原代培养的人成骨肉瘤细胞SOSP-9607的药敏感实验,研究骨肉瘤药物敏感性以及个体化治疗的可行性,从而提高骨肉瘤的治愈率。方法收集新鲜骨肉瘤组织建立SOSP-9607细胞系,以MTT比色法和流式细胞术观察5种常用化疗药对骨肉瘤细胞增殖和细胞周期时相的影响。结果骨肉瘤细胞对MTX,紫杉醇和5-FU敏感性强,生长抑制率达到51.16,49.24和42.63;流式细胞术结果显示G0/G1期细胞增多,S期细胞减少。结论骨肉瘤细胞的化疗药物敏感试验对选择敏感化疗药物开展个体化化疗,提高骨肉瘤治愈率有重要的意义。
Objeelive According to primary culture osteosarcoma cell SOSP-9607 drug sensitiveness test to study osteosarcoma drug sensitivity and improve the rate of osteosarcoma treatment. Methods SOSP-9607 cells were prepared from fresh osteosarcoma tissue. Observing the influence of five kinds of chems-drug to the cell proliferation and cell cycle of esteosareoma cell by MTT eolorimetry and flow eytometry. Results Osteosarcoma cells were more sensitive to MTX, Paclitaxe and 15-FU. The result of MTT showed the inhibitory rate were 51.16,49.24 and 42.63 respectively.The result of flow cytometry showed that G0/G1 stage cells of osteosarcoma increased and S stage ceils deceased. Coudusion The chemothempeuties drug sensitivity test of osteosareoma cells plays an important rule to improve curative effect.
出处
《中国实验诊断学》
2008年第10期1217-1219,共3页
Chinese Journal of Laboratory Diagnosis
关键词
骨肉瘤
药敏实验
细胞周期
ostesaroma
drug sensitive test
cell cycle
