淋巴生成因子VEGF-C、VEGF-D在家兔颅底组织中的表达

The expression of lymphangiogenesis factors(VEGF-C/VEGF-D) in tissue of the skull base in rabbits

目的观察家兔颅底组织:颈内动脉、硬膜和视神经中淋巴生成因子VEGF-C、VEGF-D蛋白表达,探讨家兔颅底淋巴转移途径。方法应用免疫组化染色、图像分析方法检测淋巴生成因子VEGF-C、VEGF-D在家兔颅底组织(颈内动脉、硬膜和视神经)中的蛋白表达,与脑组织中VEGF-C、VEGF-D蛋白表达比较,分析其差异。结果VEGF-C、VEGF-D在颈内动脉和硬膜中高表达,在视神经和脑组织中低表达。其中VEGF-C、VEGF-D在颈内动脉中的表达高于硬膜,二者比较,差异显著(P<0.01);硬膜高于视神经,二者比较,差异显著(P<0.01);视神经与脑组织比较,无显著性差异。结论颅底动脉、硬膜和颅底神经是颅底淋巴转移途径的重要组成部分。在家兔颅底淋巴转运过程中,颅底动脉促淋巴管生成活性最高,硬膜次之,神经组织最低。

Objective To observe the expression of lymphangiogenesis factors （VEGF-C/VEGF-D） in tissue of the skull base in rabbits,and study the mechanism of the lymph return pathway of the skull base.Methods The expression of lymphangiogenesis in tissue of the skull base,including the internal carotid artery,the dura mater and the optic nerve, were measured by using the immuno- histochemical dyeing and the image analyzed methods,compared with the expression of lymphangingenesis in the cerebral tissue of the rabbits. Results The VEGF-C/VEGF-D protein in the internal carotid artery and the dura mater were higher expression, compared with the optic nerve and the cerebral tissue. The expression of VEGF-C/VEGF-D protein inthe internal carotid artery was higher than the dum mater （ P 〈 0.01 ）, as well as the dura mater was higher than the optic nerve （ P 〈 0.01 ）. The expression of VEGF-C/ VEGF-D protein in the optic nerve had not the significance of difference,compared with the cerebral tissue. Conclusion The main cerebral artery,the dura mater and the nerve,located in the skull base,are the important role in the lymphatic transfer pathway of the skull base in rabbits. In the lymph retttm pathway of the skull base, the artery is primary key because of lymphangiogenesis ability, and the dura mater is secondary,then the nerve.