越桔品种‘达柔’离体培养快繁技术体系的研究

STUDY ON SYSTEM OF EXBODY CULTURE AND RAPID PROPAGATION OF VACCINIUM 'DARONG'

以越桔(Vacciniumssp)茎段为外植体,探讨不同植物激素ZT、、IBA、IAA的浓度对试管苗增殖以及植株再生的影响,建立一整套越桔离体培养的技术体系。实验结果表明:越桔的初代培养为改良培养基WPM+ZT 1.0mg.L-1+IAA1.0 mg.L-1+糖30 g.L-1+琼脂6.5 g.L-1,分化培养培养为改良WPM+ZT 1.0 g.L-1+IBA 0.4 mg.L-1+糖20 g.L-1+琼脂6.5 g.L-1培养基,生根培养为改良1/2WPM+IBA01.5 mg.L-1+糖20 g.L-1+琼脂6.5 g.L-1。通过正交试验也得出同样的结论。炼苗移栽时,土与蛭石(1:1)基质配比成活率最高,且生长旺盛。

Using the stem segment of Vacccinium ssp as the explant, this paper probes the effects of phytohormone of different concentration ZT, IBA, IAA on proliferation and regeneration of individual plant about cuvette seedling so that explant culture system is set up in Vaccciniurn ssp scientificaly. The results shows that the appropriate me- dium for first culture is WPM （improvved） ＋ ZT 1.0 mg ·L^-1 ＋ IAA 1.0 mg ·L^-1 ＋ sugar 30 g ·L^-1 ＋ agar 6.5 g ·L^-1 ,the medium of multiplication is WPM（improved） ＋ ZT 1.0 g ·L^-1 ＋ IBA 0.4 mg ·L^-1 ＋ sugar 20 g ·L^-1 ＋agar 6.5 g ·L^-1 ,the medium of rooting is 1/2WPM（improved） ＋ IBA 01.5 mg ·L^-1 ＋ sugar 20 g ·L^-1 ＋ agar 6.5 g ·L^-1, and same results is proved through orthogonal test . basic rate （ 1 ： 1 ） in the soil and vermiculite has high survival rate when one is planted after trainde seedling.