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不同换液程序对山羊胚胎发育的影响

Effects on Embryonic Development of Goats by Different Procedures Sequence of Changing Media
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摘要 通过优化培养程序,筛选培养液,以建立适合山羊胚胎体外发育的培养体系(in vitroculture of embryos,IVC)。培养液添加不同成分和不同换液程序的研究表明,在山羊胚胎培养中,基础培养液选用合成输卵管液(synthesized oviductal fluid,SOF)添加牛血清白蛋白(BSA)或胎牛血清(FCS),并采用正确换液程序,即48 h前是SOF+BSA,之后换为SOF+FCS,可获得理想的囊胚率和卵裂率。 This study aimed to establish a suitable culture system for IVC of goat embryos by optimizing culture procedure and screening culture medium. Studies by comparison of sequence of changing media showed that the best cultural system of goat embryos was that basal cultural media used SOF by adding BSA or FCS, and SOF plus BSA in the former stage of 48h and replacing it by FCS hereafter.
作者 吕丽华 任有蛇 李富忠 岳文斌
机构地区 山西农业大学动物科技学院
出处 《山西农业大学学报（自然科学版）》 CAS 2008年第4期425-427,共3页 Journal of Shanxi Agricultural University(Natural Science Edition)
基金 山西省自然科学基金项目(20021089) 山西省青年基金项目(20031049) 山西省攻关项目(2006031055) 山西省攻关项目(2006031066) 山西农业大学青年创新基金项目(200001)
关键词 动物繁殖 山羊 胚胎 体外培养 换液程序 Animal reproduction Goats Embryos In vitro culture Procedure of changing media
分类号 S827 [农业科学—畜牧学]
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参考文献9

  • 1Nancarrow CD, Hill JL. Co-culture, oviduct secretion and the function of oviduct-specific glycoproteins [J]. Cell Biol Int Decl. , 1994, 18 (12): 1105-1114.
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二级参考文献6

  • 1Yu Y, Li W, Han Z, et al. The effect of follicle-stimulating hormone on follicular development, granulosa cell apoptosis and steroidogenesis and its mediation by insulin-like growth factor-I in the goat ovary [J]. Theriogenology, 2003, 60 (9): 1691-704.
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共引文献3

山西农业大学学报（自然科学版）
2008年 第4期

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