幽门螺杆菌感染患儿胃黏膜上皮细胞动力学研究

Study on Dynamics of Gastric Mucosa Epithelial Cell in Children Infected by Helicobacter Pylori

目的从细胞动力学角度探讨儿童幽门螺杆菌(Hp)感染胃黏膜的致病机制。方法胃黏膜上皮细胞动力学改变采用细胞增殖及凋亡2项指标。细胞凋亡及增殖采用脱氧核糖核酸末端转移酶介导的缺口末端标记(TUNEL)技术及增殖细胞核抗原(PCNA)免疫组织化学法。对30例Hp感染胃黏膜和30例Hp阴性胃黏膜上皮细胞增殖、凋亡情况进行比较。CagA蛋白抗体检测采用Western Blot2.1免疫印迹法。结果CagA+Hp感染组30例胃黏膜上皮细胞增殖指数为(11.56±4.21)%,凋亡指数为(10.58±5.31)%。30例Hp阴性组胃黏膜上皮细胞增殖指数为(5.85±2.21)%,凋亡指数为(2.86±0.64)%。二组间增殖指数及凋亡指数比较均有显著性差异(t=7.570,8.096Pa<0.001);21例CagA+Hp感染根除治疗后,胃黏膜上皮细胞增殖指数治疗前后分别为(11.50±4.11)%和(6.30±3.26)%,具有极显著性差异(t=3.968P<0.001)。凋亡指数治疗前后分别为(10.58±4.02)%、(3.74±2.30)%,二者比较有极显著性差异(t=6.69P<0.001)。7例未根除治疗患儿胃黏膜上皮细胞增殖指数及凋亡指数变化无显著性差异(Pa>0.5)。结论Hp感染使黏膜增殖、凋亡动态失衡,黏膜的不稳定性增加,表明Hp感染是引起胃黏膜上皮细胞动力学变化失去平衡的原因之一。

Objective To explore the pathogenic mechanism of gastric mucosa epithelial cell in children infected by helicobacter pylori （Hp）. Methods Two index of cell proliferation and apoptosis had adoptted to represent the cell dynamic of gastric mucosa epithelial cell. Sixty chronic gastritis children were studied. All children underwent upper digestive tract endoscopy and biopsy specimens were taken. Hp infection from 30 Hp - positive, 30 Hp - negative cases were determined with microscopic examination with Giemsa staining and the rapid unease test. The findings with cell proliferation studied by immunostaining were correlated;The status of gastric apoptosis was tested by DNA fragmentation in situ using terminal deoxynucleotidy/transferase - mediated 2＇ - deoxyuridine 5＇ - triphosphate（dUTP） biotin nick and labeling（TUNEL）. CagA antibody was test by Western Blot 2.1. Results The proliferative index and apoptosis index in children with Hp and CagA positive gastritis including 30 cases were much higher than those of Hp negative gastritis children [ （ 11.56 ±4.21 ）% vs （5.85 ± 2.21）% ,（10.58 ±5.31）% vs （2.86 ±0.64）% ,t =7.570,8.096 Pa 〈0. 001）.There were significant differences of the proliferative index and apoptosis index in 21 Hp positive cases who had eradication treatment between before treatment and after treatment [ （ 11.50 ± 4.11）% vs （6.30±3.26）%;（10.58±4.02）% vs （3.74±2.30）%,t=3.968,6.69 Pa 〈 0. 001] . After treatment , the proliferative index and apoptosis index in 7 Hp positive cases had no significant difference compared with before treatment （ Pa 〉 0.5 ）. Conclusions Hp positive gastritis improved gastric epithelial cell proliferation and apoptosis,which indicate that Hp infection is one of the reason of cell dynamic change of gastric mucosa epithelial cell.