同型半胱氨酸对乳腺癌患者外周血单核细胞组织因子表达的影响

The Effect of Homocysteine for the Breast Carcinoma Patients' Peripheral Monocyte Expressing TF

目的探讨Hcy是否诱导乳腺癌患者外周血单核细胞(Monocyte,Mn)表达组织因子(TF)。方法用不同浓度Hcy[12.5μmol/L(Ⅱ组)、25μmol/L(Ⅲ组)、100μmol/L(Ⅳ组)、50μmol/L(Ⅴ组)]对乳腺癌患者外周血Mn进行短期培养后,粉碎细胞,ELISA方法检测其TF表达。结果随着Hcy浓度的增加,TF在乳腺癌患者外周血单核细胞的表达水平逐步增高,并呈剂量依赖性。其中Ⅰ组与Ⅲ、Ⅳ、Ⅴ组比较差异有统计学意义(P均<0.01);Ⅱ组与Ⅳ、Ⅴ组间比较,Ⅲ组与Ⅳ组、Ⅲ组与Ⅴ组间比较差异均有统计学意义;Ⅰ组与Ⅱ组、Ⅱ组与Ⅲ组、Ⅳ组与Ⅴ组间比较差异无统计学意义。结论Hcy通过诱导乳腺癌患者外周血Mn表达TF,从而在乳腺癌的侵袭和转移中发挥一定的作用。

Objective To explore the effects of homocysteine on expression of tissue factor（ TF） in peripheral monocyte in cases with breast carcinoma. Methods T The peripheral monocyte were incubated with the culturing liquid of different Hcy concentrations for short time, and then smashed.The levels of TF was detected using ELISA method.Results As incubated with Hcy, monocytic cells can be induced to express TF, which is upregulated in dose - dependent pattern. Among the groups, there is statistical difference between group Ⅰ and group Ⅲ, group Ⅰ and group Ⅳ, group Ⅰ and group Ⅴ, group Ⅱ and group Ⅳ, group Ⅱ and group Ⅴ, group Ⅲ and group Ⅴ. Conclusion Hcy induce Monocytic cells to express TF and play a rare in the invasion and metastasis of the breast carcinoma.