卤代荧光素与西布曲明相互作用的共振散射光谱特征及其分析应用

Resonance Rayleigh Scattering Study of the Interaction of Sibutramine Hydrochloride with Halofluorescein and Its Analytical Applicaiton

利用共振瑞利散射法，研究了乙基曙红、赤鲜红和荧光桃红3种卤代荧光素与盐酸西布曲明的相互作用，发现这3种卤代荧光素与盐酸西布曲明形成了离子缔合物，产生强烈的共振瑞利散射信号，其最大散射峰分别位于316、573和370nm；考察了适宜的反应条件及共存物质的影响，在一定范围内盐酸西布曲明的浓度与共振瑞利散射强度成正比，其线性范围分别是0．1～4．2mg·L^-1（乙基曙红-盐酸西布曲明体系）、0．09-6．0mg·L^-1（赤鲜红-盐酸西布曲明体系）、0．06～3．0mg·L^-1（荧光桃红-盐酸西布曲明体系），其检出限分别为32、28和21μg·L^-1；在赤鲜红-盐酸西布曲明体系中，573nm处的共振散射光谱偏振度P为0．95，表明体系的共振散射光谱主要由散射光构成。

The interactions of halofluorescein, such as ethyleosin（EE） , erythrosine （ET） and phloxine （PX）, with sibutramine hydrochloride（SH） have been investigated by resonance Rayleigh scattering（RRS）. It was found that SH could react with halofluoreseein to form ion-association complexes, resulting in a significant enhancement of RRS intensity. The RRS spectra of EE-SH, ET-SH and PX-SH complexes exhibited maximum peaks at 316, 573 and 370 nm, respectively. The optimum interaction conditions including solution pH, halofluorescein concentration, ionic strength, and formation and stabilization time have been studied. Under optimal conditions, the intensity of RRS was directly proportional to the concentration of SH in the range of 0. 1-4. 2 mg·L^-1 for EE - SH, 0. 09 -6.0 mg·L^-1 for ET-SH and 0. 06 - 3.0 mg·L^-1 for PX-SH complexes, respectively. The detection limits for SH in the system of EE-SH, ET-SH and PX-SH complexes were 32, 28 and 21μg·L^-1, respectively. The polarization（P） of RRS at 573 nm for ET-SH complex was 0. 95, indicating that the RRS spectrum of ET-SH was mainly composed of scattering light.