APC基因异常甲基化在上皮性卵巢癌中的检测及临床意义

Hypermethylation of APC Gene Promoter in Epithelial Ovarian Carcinoma

目的观察结肠腺瘤性息肉病(APC)基因异常甲基化在上皮性卵巢癌中的作用及临床意义。方法59例上皮性卵巢癌组织原发灶,32例相应的盆、腹腔转移灶,12例癌旁卵巢组织及30例正常卵巢组织,采用甲基化特异性聚合酶链式反应(MSP)法检测APC基因启动子区甲基化状态。结果上皮性卵巢癌组织原发灶及相应的盆、腹腔转移灶中存在APC基因启动子区异常甲基化,发生率分别为32.2%、40.6%,显著高于正常卵巢组织(P<0.01)。12例癌旁卵巢组织中1例检测到APC基因启动子区甲基化,30例正常卵巢组织未检测到。APC基因启动子区甲基化的发生率在临床Ⅰ、Ⅱ期低于Ⅲ、Ⅳ期(P<0.05),在高分化癌中低于低分化癌(P<0.05)。结论APC基因启动子区异常甲基化与上皮性卵巢癌发生密切相关,并可能与上皮性卵巢癌临床分期及分化程度有关。

Objective To observe the role of promoter hypermethylation of adenomatous polypesis coli （APC） gene in the course of epithelial ovarian carcinoma. Methods Hypermethylation of APC promoter was detected by methylation specific PCP （MSP） in 59 epithelial ovarian cancer primary tissues, 32 metastatic tissues of pelvic and abdomen cavity, 12 adjacent non-cancerous ovarian tissues and 30 normal ovarian tissues. Results Hypermethylation of APC promoter was detected in ovarian cancer tissues and metastatic sites, the frequency being 32.2% and 40.6% respectively, which was significantly higher than that in normal ovarian tissues（ P 〈 0.01 ）. Hypennethylation of APC promoter was detected in 1 out of 12 adjacent ovarian tissues, and no hypermethylation was detected in normal ovarian tissues. The frequency of hypermethylatiou of APC promoter was significantly lower in cancers of stage Ⅰ and Ⅱthan that in stage Ⅲ and Ⅳ （ P 〈 0.05）, and so was that in well differentiated cancers than that in poorly differentiated ones. Conclusion Promoter hypermethylation of APC plays an important role in the course of epithelial ovarian carcinoma, and it is related to clinical stage and histopathological grade in epithelial ovarian carcinoma.