利用组织芯片分析肝细胞肝癌中Caspase-9与Ki-67的表达及意义

Expression and clinical significance of Caspase-9 and Ki-67 in hepatocellular carcinoma

目的研究Caspase-9和Ki-67在肝细胞肝癌（HCC）的表达及与临床病理参数之间的关系。方法利用组织芯片技术和免疫组织化学方法检测76例HCC组织和38例正常肝组织中Caspase-9和Ki-67的表达情况。结果Caspase-9在HCC组织中的表达阳性率（55．3％）低于正常肝组织（78．9％）（P〈0．05），Caspase-9在Hcc组织的表达与组织分化有关（P〈0．05）；Ki-67在HCC组织中的表达阳性率（53．9％）高于正常肝组织（13．2％）（P〈0．01），Ki-67在HCC组织的表达与组织分化和转移有关（P〈0．05）；在HCC组织中Caspase-9与Ki-67的表达呈负相关（r=-0．300，P=0．008）。结论HCC中Caspase-9的低表达与Ki-67的高表达可能共同参与HCC中细胞凋亡与增殖的病理过程，在肿瘤的发生、发展过程中起重要作用。

Objective To observe the expression and clinical significance of Caspase-9 and Ki-67 in hepatocellular carcinoma （HCC）. Methods Expression of Caspase-9 and Ki-67 was detected by tissue microarray and immunohistochemical method in 76 HCC tissues and 38 normal liver tissues. Results The expressions of Caspase-9 in HCC tissues （55.3 % ） was significantly lower than that in normal tissues （78.9%） （P 〈 0.05 ） and was correlated with Edmondson, s histological grade of carcinoma （P 〈 0.05 ）, but there was no correlation among patients, age, gender, diameter, metastasis and HBsAg expression （P 〉0.05）. The expression rate of Ki-67 in HCC tissues （53.9%） was significantly higher than in normal liver tissues （ 13.2% ） （P 〈 0.01 ）, and was correlated with Edmondson, s histological grade and metastasis of carcinoma （ P 〈 0. 05 ）. There was no correlation among patients, age, sex and HBsAg expression （P 〉 0.05 ）. Meanwhile, expressions of Caspase-9 was negative correlated with Ki-67 （ r = -0. 300 , P = 0. 008）. Conclusion Low Caspase-9 expression and high Ki-67 expression may involved in the pathological process of cell apoptosis and proliferation, and play an important role in occurrence and progression of hepatocellular carcinoma.