氮离子注入番茄红素产生菌诱变选育的研究

Mutation of Blakeslea trispora in lycopene production by ion beams

采用了低能氮(N^+)注入技术,对发酵生产番茄红素的三孢布拉霉Blakeslea trispora(-)进行了诱变选育研究。实验结果表明N^+注入B.trispora(-)后得到较高的突变率和较广的突变谱,并通过诱变筛选得到BH3-701等4株高产菌株,经过连续五代遗传稳定性实验考察,高产菌株番茄红素的平均产量比出发菌株提高50%。其中BH3-701表现出很强的发酵积累番茄红素的能力,尤其在发酵后期更为突出,极大地提高了生产效率。

In this paper the mutation of lycopene producing strain, B.trispora（-） by use of low energy N＋ ion implantation,was studied. Experimental results show that higher mutation rate and wider mutation spectrum have been obtained after B.trispora （-） being implanted and four lycopene high yielding strains B.trispora （-） BH3-701 et al have been screened out. It has been found that the average production of lycopene increases by 50% compared with that of original strain after five passages in shaking flasks. The highest yield strain BH3-701 can accumulate lycopene in the later stage and increase production efficiency greatly.