Ang-2和VEGF在大肠癌血管生成中的作用及与其临床病理特征的关系

Expression of Angiopoietin-2 and Vascular Endothelial Growth Factor on Tumor Angiogenesis in Human Colon Cancer and Their Relationship with Clinicopathological Features

【目的】探讨促血管生成素-2(Ang-2)及血管内皮生长因子(VEGF)在大肠癌组织肿瘤血管生成中的作用及与其临床病理特征的关系。【方法】采用免疫组织化学方法检测Ang-2及VEGF蛋白在62例大肠癌组织中的表达水平,计数微血管密度(MVD)。【结果】肠癌组织中Ang-2与MVD呈正相关(r=0.7011,P<0.05);VEGF阳性表达组Ang-2和MVD明显高于阴性组(P<0.01);浸润浆膜、淋巴结转移肠癌组织中Ang-2、VEGF水平均高于无浆膜浸润、无淋巴结转移者(P<0.05);大肠癌各Dukes分期中,(C+D)期Ang-2水平和VEGF阳性表达率均高于(A+B)期(P<0.05)。【结论】Ang-2、VEGF及其相互作用对促进大肠癌血管生成,在大肠癌的侵袭和转移中可能起到重要作用。

[Objective]To investigate the role of expressions of Angiopoietin-2（Ang-2） and vascular endo thelial growth factor（VEGF） in tumor angiogenesis in human colon cancer and to analyze their relationship with clinicopathological characteristics. [Methods]Immunohistochemistry was used to examine the expression of Ang-2, microvessel density（MVD） and VEGF protein in 62 cases with colon cancer. [Results] There was a significantly positive correlation between the expression of Ang2 protein and VEGF protein（r=0997, P〈001）. The expression of Ang-2 was positively correlated with MVD in colon cancer（R1=0. 7011, P d0.05; R2=0. 754 , P〈0.05）. Furthermore, the expression of Ang-2 and MVD in positive VEGF group were sig nificantly higher than those in negative VEGF group（P〈0.01）. Higher Ang-2 and VEGF expression level were found in colon cancer cases with lamina and muscularis propria, positive lymph node metastasis. During different Dukes stages in colon caneer,Ang-2 and VEGF in A plus B stage were lower than those in C plus D stage. [Conelusion]Over-expression of Ang 2, VEGF and their interaction in human colon cancer may show higher tumor angiogenesis. Ang-2 and VEGF may play an important role in tumor transfer, progression and angiogenesis in colon cancer.