酵母基因上游转录因子结合位点分布的统计分析

Statistical analysis of the distribution of transcription factor binding sites in upstream regions of Saccharomyces cerevisiae genes

【目的】揭示真核生物基因上游转录因子结合位点的分布规律。【方法】挖掘了啤酒酵母基因组数据库（SGD）中基础域结构和β支架结构两超类（superclass）的转录因子结合在基因上游0-2000 bp的位置;将转录因子按照结构和调节基因的不同功能进行聚类,并对其组内和组间的结合位点数进行比较分析。【结果】转录因子结合位点集中分布在转录起始位点上游100-500 bp（61%）;结构差异较大的转录因子的结合位点分布差异显著（P〈0.01）;大多转录因子（19/22）在不同功能基因间结合位点分布差异不显著（P〉0.05）。【结论】转录因子结合位点分布与转录因子的结构相关性较大,而与所调控基因的功能相关性较小。推测不同家族转录因子结合位点在基因上游的分布具有特异性,有助于提供新的参数用以改进现有理论预测转录因子结合位点的方法。

【Objective】 The research was to study the distribution of these transcription factors binding sites in upstream regions of eukaryote genes.【Method】 The data of binding sites for transcription factors in the Saccharomyces cerevisiae genes database SGD database was mined and analyzed.Based on the structure of the transcription factors and the function of the genes they regulate,two superclass basic domains and beta-scaffold were formed and compared.【Result】 The results revealed the distribution of transcriptional regulatory sites and most of them lay between 100 and 500 bp upstream of the transcription start site（TSS）,showing the relationship between the binding sites,the structure of the transcription factors and the function of the genes they regulate.【Conclusion】 These results may reveal the existence of class-specific features in the sits distribution bound by each family of TFs.,which can help people make sure which region contains the transcription regulation information,and make a better algorithm for the discovery of transcription factor binding sites.