非胰岛素依赖型糖尿病患者红细胞膜ATPase活性改变及其影响因素

Alteration of ATPase Activity in the Erythrocyte Membrane and its Influencing Factors in the Patients with Noninsulindependent Diabetic Mellitus

目的探讨非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ及Ｃａ２＋－ＡＴ－Ｐａｓｅ活性改变的影响因素。方法测定７７例ＮＩＤＤＭ患者和５０例正常人血糖、总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、高密度脂蛋白胆固醇（ＨＤＬＣ）、脂质过氧化物（ＬＰＯ）、谷胱甘肽（ＧＳＨ）、糖化血红蛋白（ＨｂＡｌｃ）、超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨＰＸ）、红细胞膜Ｎａ＋－Ｋ＋－ＡＴ－Ｐａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性。ｔ检验、直线相关分析和多元逐步回归分析。结果ＮＩＤＤＭ组血糖、ＨｂＡｌｃ、ＴＣ、ＴＧ、ＴＣ／ＨＤＬＣ、ＬＰＯ显著高于对照组（Ｐ＜０．０１），ＨＤＬＣ、ＧＳＨ、ＳＯＤ、ＧＳＨＰＸ、红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋－ＡＴＰａｓｅ活性低于对照组（除ＧＳＨ的Ｐ＜０．０５外，其他Ｐ＜０．０１）；不同ＨｂＡｌｃ、ＴＣ／ＨＤＬＣ、ＧＳＨ、ＬＰＯ、ＴＣ组的红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性有显著性差别。红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性＝１２．８０＋０．２７（ＧＳＨ）－０．１２（ＬＰＯ）－０．０９（ＴＣ／ＨＤＬＣ），红细胞胞膜Ｃａ２＋－ＡＴＰａｓｅ活性＝１０８．２０＋１．?

Objective\ To study the influencing factors on alteration of ATPase activity in the erythrocyte membrane in the patients with noinsulindependent diabetic mellitus.\ Methods\ The activities of superoxide dismutase(SOD),glutathione peroxidase(GSHPX),and ATPase and the contents of glutathione(GSH) and hemoglobin Alc(HbAlc) in erythrocyte,as well as the levels of fast blood glucose (FBG),total cholesterol(TC),triglyceride(TG),high density lipoproteincholesterol(HDL C),and lipid peroxide(LPO) in plasma were assayed in noninsulindependent diabetic mellitus(NIDDM) patients and in healthy controls.\ Results\ An increase in the levels of FBG,HbAlc,TC,TG,TC/HDL C,and LPO and a decrease in the levels of HDL C and GSH and in the activities of SOD,GSHPX,and ATPase were observed in the NIDDM group.\ The levels of FBG,TC,TG,TC/HDL C,LPO,and HbAlc were higher in the patients than in the controls,those of HDL C and GSH and the activities of SOD,GSHPX,and ATPase were lower.\ Na +K +ATPase activity=12 80+0 27(GSH)-0 12(LPO)-0 09(TC/HDL C)(r=0 7036);Ca 2+ ATPase activity=108 20+1 24(GSH)-0 33(HbAlc)-0 55(LPO)(r=0 5397).\ Conclusion\ The activity of ATPase in the erythrocyte membrane was influenced by free radicals,nonenzymecatalytic saccharification,and TC/HDL in the patients with NIDDM.