白细胞介素1β对人肾小球系膜细胞LOX-1和ABCA1表达的影响

Impact of interleukin 1β on the expression of lectin-like oxidized LDL receptor 1 and ABCA1 in human mesangial cell line

目的研究白细胞介素1β（IL－1β）对人肾小球系膜细胞株（HMCL）植物血凝素样氧化低密度脂蛋白受体1（LOX－1）以及腺苷三磷酸结合盒转运体A1（ABCA1）表达的影响，及其与细胞胆同醇稳态的关系。方法实时定量PCR和Western印迹法检测IL－1β对人肾小球系膜细胞LOX－1、ABCA1表达的影响。结果人肾小球系膜细胞表达LOX－1mRNA和蛋白。IL－1β促进人肾小球系膜细胞LOX－1mRNA和蛋向表达，5μg／LIL－1β刺激细胞0～24h，LOX—1 mRNA表达于6h达高峰，为对照的6．87倍；LOX－1蛋－24h达高峰，为对照的1．88倍。IL－1β降低脂质负荷的人肾小球系膜细胞ABCA1 mRNA和蛋闩表达。5μg／LIL－1β刺激细胞0～48h，48h时ABCA1 mRNA和蛋向下降最明显，分别为对照的19．0％和50．62％。结论IL－1β促进人肾小球系膜细胞LOX－1表达，抑制ABCAI的表达，导致细胞内胆同醇的失衡，促使其变成泡沫细胞，可能加重肾小球硬化和肾病的进展。

Objective To observe the impact of IL-1β on the expression of lecfin-like oxidized LDL receptor 1 （LOX-1） and ATP-binding cassette transporter A1 （ABCAI） in human mesangial cell line （HMCL）, and its association with cholesterol homeostasis of HMCL. Methods Levels of LOX-1 and ABCA1 of HMCL induced by IL-1β were examined by using real-time PCR and Western blot. Results IL-1β up-regulated LOX-1 mRNA and protein expression. Treated with 5 μg/L IL-1β, the levels of LOX-1 mRNA and protein reached the peak after 6 h and 24 h of stimulation and were 6.87 folds and 1.88 folds of control rspectively. The expression of ABCA1 mRNA and protein of lipid-loaded HMCL was down-regulated by IL-1β. Stimulated with 5 μg/L IL-1β, the expression of ABCA1 mRNA and protein decreased to the lowest level, 19.0% and 50.62% of the baseline respectively. Conclusions The expression of LOX-1 can be up- regulated while the expression of ABCA1 can be decreased by the stimulation of IL-1β. IL-1β can enhance dyslipidemia and influence the balance of cholesterol homeostasis of HMCL.