叶酸对脑梗死大鼠星形胶质细胞GFAP表达的影响

Effect of Folate on GFAP Expression of Astrocytes in Rats with Cerebral Infarction

目的:探讨叶酸对脑梗死大鼠星形胶质细胞纤维酸性蛋白(GFAP)表达的影响。方法:将SD大鼠随机分为假手术(SO)组、缺血(IM)组、缺血+低剂量叶酸(IM+LFA)组和缺血+高剂量叶酸(IM+HFA)组。通过叶酸灌胃给药28d后制备大脑中动脉栓塞模型,采用免疫荧光双标记的方法检测各组大鼠脑缺血后脑组织GFAP的荧光强度及BrdU+GFAP双标阳性细胞密度。结果:在7d及14d4组均可见BrdU+GFAP双标阳性星形胶质细胞,缺血+叶酸组GFAP荧光强度及BrdU+GFAP双标阳性细胞密度均高于IM组及SO组,差异均有统计学意义(P<0.05或P<0.01)。结论:补充叶酸可以使星形胶质细胞GFAP表达增强,并促进神经干细胞的分化,从而对脑梗死大鼠具有保护作用。

Objective： To observe the effect of folate on the expression of glial fibrillary acidic protein （GFAP） of astrocytes in rats with cerebral infarction. Methods： Rats were randomly divided into four groups including sham operation （SO）, ischemia model （IM）, ischemia＋low folate （IM＋LFA） and ischemia＋high folate （IM＋HFA） groups. The cerebral ischemia model was induced by occlusion of middle cerebral artery after 28 days intragastric folate administration. The immunofluorescene intensity of GFAP and the density of BrdU＋GFAP in brain were detected by immunohistochemistry. Results： BrdU＋GFAP positive cells could be found in 4 groups at 7 d and 14 d. Compared with IM group and SO group, the immunofluorescene intensity of GFAP and the density of BrdU＋GFAP were significantly increased in the ischemia＋folate groups at both two time points （P 〈 0.05 or P 〈 0.01 ）. Conclusion： Folate can promote the GFAP expression and also enhance proliferation and differentiation ability of neural stem cell in rats with cerebral infarction.