NF-κB、TNF-α在淹溺大鼠脑组织中的表达及其与脑损伤的相关性

NF-κB and TNF-α Expression in the Drowning Rat Brain and the Relationship between Brain Injury

目的:探讨淡水淹溺后大鼠脑组织中细胞因子NF-κB、TNF-α的表达及其与淹溺应激引起脑损伤之间的关系。方法:Wistar大鼠70只,其中正常对照(Ⅰ组)、溺水致死(Ⅱ组)、溺水后存活(Ⅲ组)3h、6h、12h、24h、40h各10只,剥取脑组织固定、切片,HE染色后光镜下观察形态学改变;SABC免疫组化法检测各组大鼠脑组织中NF-κB、TNF-α的表达。结果:Ⅰ组脑组织结构清晰,无水肿、出血、坏死病灶;Ⅱ组脑实质疏松,小血管周隙增宽,部分神经细胞体积增大变性;Ⅲ组随溺水后存活时间的推移,脑损伤程度逐渐加重,其中24h脑水肿最重,40h个别脑实质小灶性液化性坏死。Ⅱ组脑指数明显高于Ⅰ组(P<0.05),并高于Ⅲ组,与3h、6h、12h差异有统计学意义(P<0.05)。与Ⅰ组相比,Ⅲ组6h时NF-κB表达显著增加(P<0.05);12h时TNF-α表达显著增加(P<0.05)。结论:淡水淹溺后存活大鼠发生脑损伤时NF-κB和TNF-α高表达,提示NF-κB、TNF-α与淹溺应激所致脑损伤有关。

Objective： To discuss the expression of NF—κB and TNF—α in freshwater drowning rat brain tissue and the relationship between drowning stress-induced brain injury. Methods： Wistar 70 rats were divided into normal control （group Ⅰ ）, died from drowning group （group Ⅱ ）, and survival from drowning group （groupⅢ） for 3 h, 6 h, 12 h, 24 h, 40 h with 10 rats in each group. The brain tissue were observed pathologically after staining with hematoxylin and eosin, and the expression of NF—κB and TNF—α was detect by Immunohistochemical staining method （SABC）. Results：The brain fabric was normal without edema, hemorrhage and necrosis in group Ⅰ . The brain parenchyma were loose, perivascular canal became widen, some neurocytes bulk and degenerate in group Ⅱ. In group Ⅲ, the brain injury aggravateed accompanying the lapse of survival time with extreme cerebral edema at 24 h, and colliquative necrosis in the brain parenchyma aparted at 40 h. cerebral index increased significantly in group Ⅱ than that in group Ⅰ （P 〈 0.05） and group Ⅲ especially at 3 h,12 h （P 〈 0.05）. Compared with group Ⅰ, NF—κB expression was significantly increased at 6 h, 12 h （P 〈 0.05） as well as TNF-α at 12 h （P 〈 0.05）. Conclusion： Freshwater drowning survivors have a serious brain injury with the increase of NF—κB and TNF—α. There is a correlation between NF—κB, TNF-α and drowning stress-induced brain injury.