重楼总皂苷对多发骨折-脂多糖两次打击模型大鼠血清细胞因子水平的影响

Effects of rhizoma paridis total saponins on levels of cytokines in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide

目的:探讨重楼总皂苷(RPTS)对多发骨折-脂多糖两次打击模型大鼠血清TNF-α、IL-1β及IL-6水平的影响。方法:68只Wistar大鼠随机分成5组,除空白对照组外,其余各组按多发骨折-脂多糖两次打击模型标准制模。制模成功后1h,除空白对照组和模型对照组外各组予以不同浓度的RPTS灌胃,干预后6h采血,以ELISA法检测血清中TNF-α、IL-1β及IL-6浓度。结果:大鼠血清TNF-α、IL-1β及IL-6浓度,模型对照组与空白对照组相比较明显升高(P<0.001),而RPTS各干预组与模型对照组相比较却明显下降(P<0.001),差异均有统计学意义。结论:重楼总皂苷可以降低多发骨折-脂多糖两次打击模型大鼠血清中的TNF-α、IL-1β及IL-6水平。

Objective ： To investigate the effect of rhizoma paridis total saponins （RPTS）on the levels of tumor necrosis factor alpha （TNF-α）, interleukin- 1 beta （IL- 1β ） and interleukin-6 （IL-6） in blood serum of two-hit rat model induced by multiple fractures and lipopolysaccharide. Methods： Sixty-eight Wistar rats were randomly divided into five groups. The models were made in four groups （expect the blank control group ）in accordance with the standard of two-hit animal model induced by multiple fractures and lipopolysaccharide. At 1 hour after models made,the rats in RPTS groups were given rhizoma paridis total saponins with different concentrations by intragastric administration. Six hours later, the concentrations of TNF-α ,IL-1β and IL -6 in the blood serum of all rats were detected by ELISA （enzyme linked immunosorbent assay）. Results：The concentrations of TNF-α, IL-1β and IL-6 in blood serum of rats in the model group were remarkably higher than those in the blank control group （P〈0.001） ,and these in the RPTS groups were remarkably lower than those in the model group （P〈0.001）. Conclusion： RPTS can decrease the levels of TNF-α and IL-6 and IL-1β in the blood serum of rats subjected to two-hit induced by multiple fractures and lipopolysaccharide.