十宝散促进软组织损伤修复过程中b-FGF及TGF-β_1表达的实验研究

Effects of Shibao Powder (十宝散) on promoting the expression of b-FGF and TGF-β_1 in the repair of soft tissue injuries

目的:观察中药十宝散对家兔的软组织损伤模型中创面肉芽组织b-FGF及TGF-β1表达的影响,从分子水平探讨中药外用制剂促进软组织修复的机制。方法:采用经典的全层皮肤切割伤造模,用8%硫化钠脱去术野皮肤,常规消毒,硫苯妥钠30mg/kg腹腔内麻醉。剪开皮肤,钝性剥离以暴露腓肠肌,在距肌腱止点1/3处用手术刀做不完全锐性切割,做一长0.8cm,宽0.4cm的切口,压迫止血。治疗组外敷十宝散并包扎,清洁换药组常规清洁换药,空白组仅换敷料。收集不同时期的肉芽组织,高倍镜下进行病理切片观察,用SABC法检测创面中b-FGF及TGF-β1蛋白表达,在400倍光镜下观察,BI-2000图像分析系统分析,结果以平均灰度值表示。结果:在同一取材的条件下,3组b-FGF蛋白表达在创伤后6d存在差异(P<0.05),TGF-β1蛋白表达在创伤后10、14d存在差异(P<0.05)。同时,肉芽组织的生长状况在同一时期取材时间的条件下存在组间不同,在不同取材时间的条件下存在组内差异。结论:本实验证明中药十宝散能够通过刺激创面内细胞产生内源性生长因子,进而促进创面的修复。

Objective：To study the effects of Shibao Powder（十宝散） on the expression of b-FGF and TGF-β1 in granulation tissues of rabbit models with soft tissue injuries, so as to explore mechanism of external Chinese drugs for repairing of soft tissues in molecular levels. Methods ：The rabbit models were established by classical method of full-thickness skin wounds. After 8% sodium sulfide was used and routine disinfection completed ,intra-peritoneal anaesthesia was adopted. The skin was cut to expose gastrocnemius muscle, and imcomplete sharp dissection was made near the tendon insertion. The length of the incision was 0.8 cm and the width was 0.4 cm. The rabbits in the experimental group were treated with spread of Shibao Powder （十宝散 ） at the wound;the rabbits in clean group were treated with routine dressing changes and disinfection;and the rabbits in the control group were treated with dressing changes only. The granulation tissues in different stages were collected and observed with high power microscope. The expression of Transforming Growth Factor-β1 （TGF-β1） protein and b-FGF protein in wound tissues were detected using StreptA-ridin-Biotin-Complex （SABC） method. Results：The results showed that the expression of b-FGF protein had significant differences among 3 groups at the 6th day after trauma（P〈0.05 ）. The TGF-β1 protein expression also had significant differences among 3 groups at the 10th and 14th days after trauma （P〈0.05）. At the same time, the growth states of granulation tissues had difference among 3 groups at the same trauma stage, and within the same group among different trauma stages. Conclusion ：This experimental study shows that Shibao Powder （ 十宝散 ） is effective to promote the repair of soft tissues after trauma by stimulating production of endogenous growth factor from cells in wound.