TNF-α抑制剂对重症急性胰腺炎细胞凋亡及Caspase-3表达的影响

Effects of tumor necrosis factor-α inhibitor on cell apoptosis and expression of Caspase-3 in rats with severe acute pancreatitis

目的:研究重症急性胰腺炎(SAP)大鼠细胞凋亡、Caspase-3表达及其与疾病严重程度的关系.方法:40只SD大鼠随机分成对照组(SAP组,胆管逆行性注射牛磺胆酸钠制备SAP模型,n=20),实验组(干预组,SAP模型制作成功后,注射重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白,n=20).测定两组血清淀粉酶和TNF-α含量,采用免疫组化法分析Caspase-3的表达,运用TUNEL技术检测胰腺细胞的凋亡,并对胰腺组织进行病理学评分.结果:与SAP组比较,干预组的血清淀粉酶、TNF-α、胰腺组织病理学评分较低(6868.80±595.50U/Lvs5434.20±481.52U/L,258.46±38.57ng/Lvs182.00±19.67ng/L,15.54±2.30vs11.06±1.09,均P<0.05),Caspase-3阳性率和和胰腺细胞的凋亡指数显著升高(20.06±2.17vs28.52±3.74,10.42±1.27vs16.23±2.43,均P<0.05).结论:SAP时胰腺细胞发生凋亡时伴随Caspase-3的高表达,而炎症反应和胰腺组织病理学评分得到改善.

AIM： To investigate the relationship between the pancreatitis cell apoptosis, Caspase-3 expression and disease severity in severe acute pancreatitis （SAP） rats. METHODS： Forty SD rats were randomly divided into SAP group treated with retrograde injection of sodium taurocholate （n = 20）, and experimental group （intervention group, SAP animal model was developed, the recombinant human necrosis factor-alpha receptor II： IgG Fc fusion protein was injected, n = 20）. Serum amylase and TNF-α were detected. And Caspase-3 expression was measured using immunohistochemitry. And the apoptosis of pancreatic cells were detected using TUNEL and the pathological score of pancreatic tissue was estimated. RESULTS： Serum amylase, TNF-α and pathological scores of pancreatic tissues were lower （6868.80 ± 595.50 U/L vs 5434.20 ± 481.52 U/L, 258.46 ± 38.57 ng/L vs 182.00±19.67 ng/L, 15.54 ± 2.30 vs 11.06 ± 1.09, all P 〈 0.05）, and the positive rate of Caspase-3 and the apoptotic index of pancreatic cells increased significantly in the intervention group than in the SAP group （20.06±2.17 vs 28.52 ± 3.74, 10.42± 1.27 vs 16.23 ± 2.43, both P 〈 0.05）. CONCLUSION： Pancreatic cell apoptosis is associated with increased expression of the Caspase-3, improved the inflammatory response and lowered pancreatic tissue pathological score in rats with severe acute pancreatitis.