溃疡性结肠炎患者血浆MMP-1与TIMP-1测定的意义

Clinical significance of plasma MMP-1 and TIMP-1 measurement in patients with ulcerative colitis

目的:检测溃疡性结肠炎(ulcerative colitis,UC)患者血浆基质金属蛋白酶-1(MMP-1)及其组织抑制因子-1(TIMP-1)水平.方法:采集经临床表现、肠镜及病理诊断的UC患者30例以及正常人15例的外周静脉血,ELISA法测定血浆MMP-1和TIMP-1水平.结果:UC患者血浆MMP-1、TIMP-1水平明显高于对照组(2.4421±0.5394vs1.8967±0.3737,6.3728±0.4940vs5.5917±0.2968,均P<0.05);UC患者血浆TIMP-1水平与病情严重程度呈正相关(t=4.097,P<0.05),而血浆MMP-1水平与病情严重程度无相关性;中-重型患者血浆MMP-1水平明显升高,而轻型患者血浆MMP-1的水平较对照组无明显的差别.结论:UC患者血浆中MMP-1与TIMP-1呈现高表达;血浆MMP-1,特别是TIMP-1可能成为判断UC严重程度以及临床诊断简单易行的外周血生物学指标.

AIM： To determine the plasma level of matrix metalloproteinase-1 （MMP-1） and its tissue inhibitor of metalloproteinase-1（TIMP-1） in patients with ulcerative colitis （UC） and to investigate their clinical significance. METHODS： ELISA assay was used to determine the plasma MMP-1 and TIMP-1 level in the peripheral venous blood samples from 30 pathologically confirmed UC patients and 15 normal healthy controls. RESULTS： Plasma levels of MMP-1 and TIMP-1 were significantly higher in patients with UC than in normal controls （2.4421 ± 0.5394 vs 1.8967 ± 0.3737, 6.3728 ± 0.4940 vs 5.5917 ± 0.2968, both P 〈 0.05）; Plasma level of TIMP-1 was positively correlated with the severity of the disease （t = 4.097, P 〈 0.05）, but that of MMP-1 wasn＇t; Plasma level of MMP-1 in moderate to severe patients was significantly higher than that in controls, but no significant difference was found between mild patients and control groups. CONCLUSION： Plasma level of MMP-1 and TIMP-1 are significantly elevated in patients with UC; Plasma MMP-1, particularly plasma TIMP-1 could be considered as simple biological parameters for the severity and clinical diagnosis of UC.