参数自调整非接触式微量试剂分配系统

Non-contact submicroliter liquid dispensing system with parameter adjustment function

研制了一款具有控制参数自调整功能的非接触式微量试剂分配系统,该系统采用精密微电磁阀控制预压液体的流动来实现试剂的分配操作。独特的试剂分配管路设计使电磁阀不易堵塞;非接触式的分配方式减少了试剂污染,分配速度更快,更易清洗。研制了一款MEMS压差式微流量传感器并集成于系统中用于采集试剂分配过程中的流量信息,实现了试剂特性和体积变化时控制参数的自动调整,提高了分配精度和灵活性。针对不同粘性试剂进行分配精度的测试,结果表明,该系统最小可分配50 nl体积试剂,分配精度达8%。在分配试剂体积>1μl时,重复精度<3%。该试剂分配系统可在高通量试剂操作过程中,针对多种不同特性试剂进行快速、灵活、微量、精确的试剂分配操作。

An non-contact submicroliter liquid dispensing system with parameter adjustment function was developed. The system used a solenoid valve to control fluid flow to realize the noncontact reagent dispensing by opening and closing a valve and altering opening time and flow rate. By combining the advantages of non-contact solenoid-based technology with the robustness and flexibility of traditional liquid handlers, a novel high-speed flow MEMS sensor was proposed,it is characterized by robustness, repetition, flexibility and low-volume dispensing for samples with different properties. The experimental results indicate that the dispense reproducibility（Coefficient of Variance,CV） of the proposed system is lower than 3% at dispensing volume of 1μl,and approach to 8% at dispensing volume of 50 nl, which can satisfy the requirements of dispensing submicroliter reagents in different viscosities, repetitions, flexibilities and reliabilities.