镉诱导小鼠睾丸细胞凋亡与Caspase-3、Bcl-2和Bax mRNA的表达

Apoptosis Induced by Cadmium and the Expression of Caspase-3,Bcl-2 and Bax mRNA in Testis of Mouse

一个月大雄性小鼠24只,随机分为6组,用30μmol/kgCdCl2作用小鼠睾丸不同的时间(3h、6h、12h、18h、24h)后,利用DNA电泳、免疫组化和半定量RT-PCR技术,分析生殖细胞凋亡过程中三种关键物质Caspase-3、Bcl-2、Bax蛋白和mRNA的表达量变化。结果显示:1)DNA各组(除对照组外)均出现不同程度断裂。2)Caspase-3蛋白表达量一直上升,与对照组相比差异极显著;Bax蛋白在12h前一直上升,与对照组相比差异显著,12h后又开始下降,且与对照组相比无显著差异;Bcl-2蛋白在下降,与对照组相比差异显著。3)RT-PCR结果显示Caspase-3基因表达量减少;Bax基因表达量逐渐上升;Bcl-2基因表达量波动很大。综上所述,Caspase-3、Bcl-2和Bax三个基因可能参与了镉应激状态下小鼠睾丸组织细胞的凋亡过程。

Juvenile （ 1 month old） mice were randomly divided into 6 groups. DNA electrophoresis, immunohistochemistry and semi-quantity RT-PCR methods were used to investigate the proteins and mRNA expression of Caspase-3, Bax and Bcl-2 which were involved in the apoptosis of mouse testis. The results were shown as follows ： 1 ） DNA departed at all the groups except the control. 2 ） The expression of Caspase-3 protein was up-regulated and the difference with control was significant. The expression of Bax protein was up-regulated in the 3 h, 6 h and 12 h groups and down-regulated in 18 h, 24 h groups. The expression of Bcl-2 protein was down-regulated at all the groups. 3 ）The result of RT-PCR showed that, Caspase-3 gene was slightly down-regulated, Bax gene was obviously up-regulated, but the Bcl-2 expression was greatly fluctuant. It was shown that Caspase-3, Bax and Bcl-2 gene maybe involved in the mouse testis apoptosis which induced by cadmium.