^(60)Co-γ射线辐照降解的壳聚糖对玉米萌发及生长的影响(英文)

The Effect of the Degradation Gradients of Chitosan Irradiated by ^(60)Co-γ Ray on the Maize Germination and Seedling Growth

采用不同剂量60Co-γ射线辐照降解壳聚糖,研究了其降解产物对玉米生长的调节作用。研究发现,壳聚糖辐照产物可提高玉米种子发芽率,促进玉米幼苗生长,提高玉米抗逆相关生理生化指标。辐照剂量为200kGy,壳聚糖辐照产物溶液浓度为3.0mg/mL时,可显著提高玉米种子萌发率和促进芽长;浓度为4.0mg/mL时,显著促进主根长度;浓度为2.0mg/mL^3.0mg/mL,苯丙氨酸解氨酶(PAL)活性显著高于对照。当辐照剂量为200kGy^400kGy,产物溶液浓度为2.0mg/mL^4.0mg/mL时,能显著提高胚乳中α-淀粉酶活性和幼苗中叶绿素含量;浓度为2.0mg/mL^3.0mg/mL时,显著提高幼苗过氧化物酶(POD)的活性。综合多种影响因素得出:利用壳聚糖辐照产物开发玉米促生抗逆剂的适宜60Co-γ射线辐照剂量为200kGy^300kGy,浓度为2.0mg/mL^3.0mg/mL。

The chitosan was irradiated by ^60Co-γ ray at different dose, and then studied the degradations of chitoson to regulate maize growth. The results showed that chitosan degradation ingredients can enhance seed germination rate, raise corn resilience and resistance. At the irradiation dose of 200 kGy, the seed germination rate and the sprouts length were significantly higher than the controls at the concentration of 3.0 mg/mL, the length of taproot was significantly higher than the controls at the concentration of 4.0 mg/mL, and the peroxidase（PAL） activity of maize seed was significantly higher than the controls at the concentration of 2. 0 mg/mL - 3.0 mg/mL. At the doses of 200 kGy- 400 kGy, the α- amylase activity and the chlorophyll were significantly higher than the controls at concentrations of 2.0 mg/mL - 4. 0 mg/mL, the peroxidase（POD） activity of maize seed was significantly higher than the control at concentration of 2.0 mg/mL - 3.0 mg/mL. Considering these results and cost of production, we explored resistance reagent on maize, the appropriate ^60Co-γ ray irradiation doses was 200 kGy - 300 kGy, and the concentrations was 2.0 mg/mL - 3.0 mg/mL.