阿尔茨海默病患者脑脊液细胞因子研究

Study on the level of cytokine in cerebrospinal fluid in patients with Alzheimer's disease

目的:探讨细胞因子在阿尔茨海默病(AD)发病机制中的意义和临床诊断价值。方法:采用双抗体夹心酶联免疫吸附试验分别对25例患者脑脊液(CSF)白细胞介素1β(IL-1β)、白细胞介素6(IL-6)及肿瘤坏死因子α(TNF-α)水平检测。结果;AD患者CSFIL-1β、IL-6和TNF-α含量均较正常老年人显著为高(P均<0.01)。结论;AD患者CSFIL-1β、IL-6和TNF-α含量较正常老年人显著增高,为AD的慢性免疫炎症机制提供了理论依据。

Objective： To investigate the pathogenesis meaning and clinical diagnosis value of cytokine in Alzheimer＇s disease （AD） Method：25 patients with Alzheimer＇s disease were included. Commercial ELISA adsorption test was used to investigate the levels of interleukin-1β （ IL-1 β ） , interleukin-6 （ IL-6 ） and tumor necrosis factor ct（TNF-α） in CSF of the patients. Results：The average CSF level of IL-1β,IL-6 and TNF-α was significantly higher in AD patients than in healthy controls （ P 〈 0.01 ）. Conclusion： These results strongly suggested that inflammatory cytokines IL-1β ,IL-6 and TNF-α should be important factors in the neuro- degeneration process associated with AD and the inflammation should significantly contribute to the pathogenesis of AD.