附子提取物对移植性肝癌H_(22)细胞凋亡影响的实验研究

Effect of Radix Aciniti Praeparata Extract on Cell Apoptosis in Transplanted Liver Cancer H_(22)

目的:研究附子提取物抗移植性肝癌H22的作用机制,并探讨与环磷酰胺是否具有增效协同作用。方法:建立移植性肝癌H22模型,随机分组,腹腔注射给药,观察各组抑瘤效应,检测TNF-α、NF-κB及Caspase-3表达。结果:附子提取物能显著抑制肝癌移植瘤的生长(P<0.05),与环磷酰胺合用可显著抑制移植瘤生长(P<0.01),有协同作用(q>1);附子提取物可显著促进移植瘤TNF-α和Caspase-3表达,抑制NF-κB表达(P<0.05),合用环磷酰胺可极显著促进TNF-α和Caspase-3表达、抑制NF-κB表达(P<0.01)。结论:附子提取物具有确切的抑瘤作用,其效应机制可能与活化细胞凋亡信号传导通路,诱导肿瘤细胞凋亡有关,并与环磷酰胺有协同增效作用。

Objective：to study the functional mechanism of Radix Aconiti Praeparata Extract against transplanted liver cncer H22,and probe into its synergistic interactions with cyclophosphamide.methods：transplanted liver cancer H22 model were established and randomly diveided into different groups.After the intraperitoneal injection,observe the tumor-inhibiting effect of each group,detect the expressions of TNF-α,NF-κB and Caspase-3,Results：The Radix Aciniti Praeparata Extract can obviously inhibit the growth of transplanted tumor（P〈0.01）,with synergistic effect（q〉1）,Radix Aconiti Praeparata Extract can obviously improve the expressions of transplanted tumor TNF-α and caspase-3,inhibit the expression of NF-κB （P〈0.05）,its application together with cyclophospharmide can significantly improve the expressions of TNF-α and caspase-3,inhibit the expression of NF-κB（P〈0.01）,Conclusion：Radix Aconiti Praeparata Extract has exact tumor-inhibiting effect,its effect machanism may be related to the transmission passway of the activated cell apoptosis signal and tumor cell apopto sis induction,and have synergistic effect with eyclophosphamide.