联合应用IL-2基因疗法和IL-3基因疗法对骨髓移植后荷瘤小鼠免疫功能的增强作用

THE AUGMENTATION OF IMMUNE FUNCTION OF TUMORBEARING MICE BY IL2 GENE THERAPY COMBINED WITH IL3 GENE THERAPY AFTER BONE MARROW TRANSPLANTATION

以大剂量化疗后给予同基因骨髓移植（ＢＭＴ）的荷瘤小鼠为实验模型，动态观察了联合应用ＩＬ－２基因疗法和ＩＬ－３基因疗法配合ＢＭＴ对荷瘤小鼠抗肿瘤免疫功能的影响。结果表明，联合应用基因疗法能显著提高ＢＭＴ治疗后荷瘤小鼠脾细胞诱导的ＣＴＬ杀伤活性、腹腔巨噬细胞杀伤活性及其所分泌的ＩＬ－１、ＴＮＦ水平，但是对荷瘤小鼠脾脏分泌ＩＦＮ－γ无协同诱导效应。提示联合应用ＩＬ－２基因疗法和ＩＬ－３基因疗法通过显著地协同促进机体某些免疫功能恢复而增强ＢＭＴ后的抗肿瘤作用。

 The authors investigated the effects of IL2 gene therapy and IL3 gene therapy on the antitumor immune functions of tumorbearing mice which received high dose chemotherapy and then syngeneic bone marrow transplantation(BMT).The results showed that the combined use of the IL2 gene therapy and IL3 gene therapy significantly augmented the cytotoxicity of CTL and peritoneal macrophages of tumorbearing mice after BMT and markedly increased the levels of IL1 and TNF secreted by the macrophages.These results suggested that the combined use of IL2 gene therapy and IL3 gene therapy could augment the antitumor effects of BMT more significantly by efficient promotion of immune reconstitution.