维药伊木萨克片对大鼠性功能与阴茎组织中一氧化氮合酶活性的影响

The effect of Uygur medicine Yi-Mu-Sa-Ke on the sexual ability and the NOS activity of male rats

目的:探讨维药伊木萨克片对雄性大鼠阴茎勃起功能的影响及其机制。方法:取具有正常性功能SD雄性大鼠20只,随机分为正常对照组(n=10)和伊木萨克组(n=10),用等量溶剂与伊木萨克片(250mg·kg-1.d-1)分别对2组大鼠连续干预6周后,进行阿朴吗啡(APO)阴茎勃起实验和交配实验,并检测阴茎海绵体窦压(ICP);用酶联免疫吸附试验(ELISA)法检测阴茎组织中的一氧化氮合酶(NOS)活性。结果:(1)与正常对照组比较,伊木萨克组阴茎勃起所需时间显著缩短(P<0.01),插入次数明显增加(P<0.01),且基础ICP值与电刺激诱导ICP值均显著升高(P<0.05);(2)NOS活性在伊木萨克组显著高于正常对照组(P<0.01)。结论:维药伊木萨克片能够显著提高正常大鼠的阴茎勃起功能,其机制与提高NOS活性有关。

Objective： To study the effect of Uygur medicine Yi-Mu-Sa-Ke on the penis erectile function of male rats. Methods： Twenty male Sprague-Dwaley rats were randomly divided into two groups： the exper- imental control group and the Yi-Mu-Sa-Ke drug group. Uygur medicine Yi-Mu-Sa-Ke were given 250 mg/ kg gavages, for six weeks. In the sixth week of the two groups were conducted APO penis erectile experiments and mating test. Then decteted the two groups＇ intra cavernous pressures （ICP）. Results： （1）The need time of penis erectile in the Yi-Mu-Sa-Ke drug group shortened than the control group （P 〈0.01） and the number of mating insert increased （P 〈0.01）. Besides,the basic ICP value and the induced ICP（electrical stimulation）were higher than the normal group （P 〈0.05）. （2）The activity of NOS in the Yi-Mu-Sa- Ke drug group was higher than the control group. Conclusion： The study confirmed that Uygur medicine Yi-Mu-Sa-Ke can significantly improve the erectile capacity of normal rats and the mechanism of it may be the raise of NOS.