新辅助化疗对直肠癌细胞凋亡与增殖的影响

Affection of neoadjuvant chemotherapy on cell apoptosis and proliferation in patients with rectal carcinoma

目的:探讨新辅助化疗(FOLFOX方案)对直肠癌细胞凋亡与增殖的影响。方法:选择进展期直肠癌患者76例,随机分为两组:常规手术组和新辅助化疗+手术组,各38例。分别应用TUNEL法和免疫组织化学染色法检测直肠癌细胞的凋亡(AI)与增殖(PI),并计算二者之间的比值(AI/PI)。结果:新辅助化疗+手术组与单纯手术组AI、PI差异具有统计学意义(P<0.01),AI/PI差异有统计学意义(P<0.01)。结论:新辅助化疗(FOLFOX方案)可明显促进直肠癌细胞凋亡并抑制其增殖。

Objectives： To investigate cell apoptosis and proliferation effect of neoadjuvant chemotherapy （FOLFOX） in patients with rectal carcinoma. Methods： Seventy-six cases with advanced rectal cancer were randomly divided into routine surgical operation group （RSO） and neoajuvant chemotherapy-r surgical operation group （NCSO）. Apoptosis （AI） and proliferative index （PI） of rectal cancer cells were detected by using the TUNEL and immuno-histochemical staining, and caculated ratio of AI and PI. Results： There were statistical significance of AI and PI between the Group RSO and the Group NCSO （P〈0.01）, and there was statistical significance of AI/PI between them too （P〈0.01）. Conclusions： Neoadjunvant chemotherapy （FOLFOX） can markedly enhance apoptosis of rectal cancer cells and inhibites their proliferation.