离子注入对雪松花粉粒萌发与花粉管生长的影响

EFFECTS OF ION BEAM IMPLANTATION ON POLLEN GERMINATION AND POLLEN TUBE GROWTH OF Cedrus deodara

用激光扫描共聚焦显微技术(LSCM)研究了低能离子注入对雪松花粉管萌发和生长的影响。结果表明,低剂量(1×1015ions/cm2)注入对雪松花粉粒萌发没有明显的影响;3×1015ions/cm2和5×1015ions/cm2离子注入可以刺激花粉粒萌发;高剂量(等于或大于7×1015ions/cm2)的离子注入会显著抑制花粉粒萌发。离子注入对雪松花粉管的伸长和花粉管的形态特征有比较明显的影响,花粉管受到的损伤程度与离子注入剂量有一定的相关性。

Effects of ion beam implantation on pollen germination and pollen tube growth of Cedrus deodara were investigated by the laser confocal microscopy technique（LSCM）. The results showed that the low close ion implantation （1 × 10^15 ions/cm^2） did not affect the germination of the pollen. The doses of 3 × 10^15 ions/cm^2 and 5 × 10^15 ions/cm^2 implantation could significantly promote the germination. But the high dose ion implantation （equal to or over 7 × 10^15 ions/cm^2） obviously suppressed the pollen to germinate. The effects on elongation and morphological characters of the pollen tube caused by the ion beam implantation were observed. The damage degree of the pollen tube increased with the increase of ion implantation dose.