维奥欣对实验性动脉粥样硬化大鼠C反应蛋白的影响

The Effect of Wei Ao Xin on C-Reactive Protein in the Experiment Atherosclerosis Rats

目的:观察维奥欣对实验性动脉粥样硬化(AS)大鼠C反应蛋白(CRP)的影响,探讨其在AS中是否存在抗炎作用。方法:60只雄性SD大鼠随机分为5组:假手术组、模型组、阿斯匹林组、维奥欣小剂量组、维奥欣大剂量组,每组12只,各组予以相应药物治疗。3个月后,处死动物,留取标本,测定血脂,并进行颈总动脉血管病理学观察及CRP多克隆抗体免疫组化分析。结果:与模型组比较,维奥欣大剂量组血清TG、TC、LDL-C水平显著下降(P<0.05),且明显低于阿斯匹林组(P<0.05)。维奥欣小剂量组与模型组比较无明显差异(P>0.05)。与假手术组相比,模型组损伤侧颈动脉内膜明显增厚,局部有斑块形成,W/L明显增大(P<0.01)。维奥欣大、小剂量组W/L明显小于模型组(P<0.01,P<0.05),维奥欣大剂量组低于阿斯匹林组(P<0.05),维奥欣小剂量组与阿斯匹林组间无显著性差异(P>0.05)。免疫组化染色结果显示,与模型组比较,维奥欣大、小剂量组CRP表达水平显著下降(P<0.01,P<0.05),维奥欣大剂量组明显低于阿斯匹林组(P<0.05),而维奥欣小剂量组与阿斯匹林组间比较无显著性差异(P>0.05)。结论:维奥欣可通过降脂、抗炎等作用减缓实验性动脉粥样硬化大鼠内膜增生,延缓动脉粥样硬化进程。

Objective：To observe the effect of Wei Ao Xin on C-reactive protein （CRP）of experiment atherosclerosis （AS） rats, and its anti-inflammatory action on AS. Methods：Sixty male SD rats were assigned randomly into five groups：group of sham operation,group of model,group of aspirin,group of Wei Ao Xin of small dosage （WAXs） ,and group of Wei Ao Xin of large dosage （WAX1）,with 12 in each group. Serum triglyceride （TG）, cholesterol （TC）,low density lipoproteincholesterol （LDL-C）,high density lipoprotein-cholesterol （HDL-C）and CRP were detected three month later. Results： Compared with group of model, the level of serum TG, TC and LDL-C in group of WAX1 were significantly decreased （P〈 0.05） ,and lower than that in group of aspirin （P〈0.05）. However,there was no significant difference between WAXs and the model group （P〉0.05）. The level of serum HDL-C was not significantly different between the treated groups and the model group （P〉0.05）. In the impaired side,W/L ratio of carotid artery in the model group was thickened obviously than the sham operation group （P〈0.01）and locally formed plaque. The W/L ratio of carotid artery in the large and small dose Wei Ao Xin-treated group was much thinner than that of model group（P〈0.01 ,P〈0.05）. That of WAX1 group was thinner than aspirin-treated group（P〈0.05）. The W/L ratio of carotid artery in the small dose Wei Ao Xin-treated group was much thinner than that of model group（P〈0.05）. But there was no significant difference between WAXs group and the aspirintreated group （P〉0.05）. Immunohistochelnistry staining showed that compared with the model group,CRP in WAX1 and WAXs groups were significantly decreased （P〈0.01 ,P〈0.05）. That of WAX1 was significantly lower than that of aspirintreated group（P〈0.05）. CRP in WAXs group were significantly decreased （P〈0.05）. But there were no significant difference between WAXs group and the aspirin-treated group （P〉0.05）. Conclusion：Wei Ao Xin can reduce the endomembrane hyperplasy in atherosclerosis rats through its anti-inflammatory action.