康莱特诱导肝癌细胞HepG2凋亡及其对Bcl-2、Caspase-8表达影响的研究

The effect of KLT on apoptosis of HepG2 cells and expression of Bcl-2 and Caspase-8

目的观察康莱特（Kang-Lai—Te）对HepG2细胞调亡及其对Bcl-2和Caspase-8表达的影响。方法将不同浓度的康莱特作用于人肝癌细胞HepG2，并于12、24、48h后收集HepG2细胞。同时设立空白对照组（未加康莱特组）。流式细胞术检测各实验组及对照组HepG2细胞的凋亡率，并检测Bcl-2及Caspase-8的表达量。结果康莱特能明显诱导人肝癌HepG2细胞的凋亡，随时间延长凋亡率增加，并可明显增加Caspase-8的表达量，而对Bcl-2的表达影响不大。结论康莱特可能通过调节Caspase-8的表达来诱导HepG2细胞的凋亡。

Objective To investigate the effects of KLT on apoptosis of HepG2 cells and expression of Bcl-2 and Capase-8. Methods The cell line HepG2 was induced by diverse density of KLT, and HepG2 cell was collected respectively after induction of 12 h, 24 h, 48 h. The control group was installed simultaneouly. The cell apoptosis and the expression of Bcl-2 and Caspase-8 were detected by flow cytometry （FCM）. Results KLT can induce the apoptosis of HepG2 cell significantly, and the longer time past, the more apoptosis of HepG2 was. KLT can increase the expression of Caspase-8, but ineffective to Bcl-2. Conclusion KLT can significantly induce the apoptosis of HepG2 cell through regulating the expression of Caspase-8.