大鼠原位肝脏隔离灌注大剂量5-Fu的病理形态学观察

THE PATHOLOGICAL OBSERVATIONS OF ISOLATED LIVER PERFUSION WITH LARGE DOSE 5-FU IN RATS IN VIVO

应用大鼠原位肝脏隔离灌注技术行不同剂量５－Ｆｕ肝脏灌注，观察肝脏的病理形态学变化。结果：正常肝组织可耐受≤２５０ｍｇ／ｋｇ的５－Ｆｕ区域灌注，５－Ｆｕ剂量为１５０ｍｇ／ｋｇ时，肝脏超微结构为可逆性改变。此种变化与灌注液无５－Ｆｕ组、全肝血流阻断组无显著差异。结论：在允许的剂量范围内行肝脏隔离灌注５－Ｆｕ并不加重肝组织的缺血再灌注损伤。

Isolated liver perfusion (ILP)in rats in vivo using different doses of 5 Fu was performed to observe the pathological changes of the liver.The results showed that the normal hepatic tissue could tolerate regional perfusion with no over than 250mg/kg 5 Fu.The changes of hepatic ultrastructure were reversible in ILP with 150mg/kg 5 Fu which had no significant differences compared with those of ILP without 5 Fu and total hepatic vascular exclusion.From the above it can be seen that ILP within 5 Fu of the maximal tolerance does not deteriorate ischemia reperfusion injuries of hepatic tissue.