摘要
为探讨ｖ－ｓｉｓ表达在脑肿瘤中的作用，我们用Ｄｉｇ标记探针，用原位杂交结合图像分析的方法对２８例脑膜瘤和３０例胶质瘤中的ｖ－ｓｉｓｍＲＮＡ表达进行定位、定量分析。结果表明：ｖ－ｓｉｓｍＲＮＡ在脑膜瘤和胶质瘤中的阳性表达率分别为６０．７％和７０％。经ｔ检验，两种脑瘤中ｖ－ｓｉｓ表达明显高于正常脑组织中的表达（Ｐ＜０．０１）。说明ＰＤＧＦ／ＰＤＧＦＲ自泌刺激环路在脑瘤的发生、发展中具有重要作用。
To investigate the function of v sis expression in brain tumors,we studied the mRNA expression of v sis in 28 meningiomas and 30 gliomas using in situ hybridyzation and image analysis methods with Dig labeled probe.The results showed that the positive expression rates of v sis mRNA in meningiomas and gliomas were 60.7% and 70% respectively.The v sis expressions in the two brain tumors were much higher than those in normal brain tissues(p<0.01).These suggested that the autocrine loop of PDGF/PDGFR played an important role in pathogenesis and development of brain tumors.
出处
《滨州医学院学报》
1997年第4期317-318,共2页
Journal of Binzhou Medical University
关键词
v-sis
脑肿瘤
Dig标记
原位杂交
v sis
brain tumors
Dig label
in situ hybridyzation
image analysis
