美洛昔康对人肝癌裸鼠原位移植瘤生长的抑制性影响的研究

THE INHIBITORY EFFECT OF MELOXICAM ON THE GROWTH OF HUMAN HEPATOCELLULAR CARCINOMA IMPLANTS IN NUDE MICE

目的研究选择性COX-2抑制剂美洛昔康对人肝癌裸鼠原位移植瘤生长的影响并初步探明其机制。方法建立裸鼠人肝癌原位移植瘤模型,给予美洛昔康治疗8周,观察其对裸鼠体内肝癌生长的影响。采用TUNEL法观察细胞的凋亡;采用免疫组化法检测美洛昔康对肝癌组织中COX-2、增殖细胞核抗原(PCNA)、P53表达的影响。结果美洛昔康可抑制裸鼠人肝癌原位移植瘤生长,其抑瘤率为65.3%,还能明显诱导肝癌细胞凋亡,TUNEL所测凋亡指数为16.90±3.60,与对照组(5.98±0.94)比较,P<0.01;美洛昔康能明显抑制肝癌组织中COX-2、PCNA表达,并促进P53表达。结论体内实验表明,美洛昔康能有效抑制人肝癌生长并诱导凋亡。

Objective To investigate the effects of the selective COX-2 inhibitor meloxicam on the growth and apoptosis of hepatoeellular carcinoma in vivo. Methods The animal model of xenotransplanted tumors in nude mice was established. Meloxicam was administered for 8 weeks in order to observe its effects on the growth of liver cancer implants in nude mice. Apoptosis of HCC was observed by TdT-mediated dUTP nick end labling in situ assay （TUNEL） . Immunohistochemistry was used to detect the expression of COX-2, proliferating cell nuclear antigen （PCNA）, and P53 in liver cancer tissue. Results Meloxicam significantly inhibites the growth of implanted liver cancer, the inhibition rate being 65.3 %. It also induced the apoptosis of HCC cells, with a higher apoptotic index （AI） （16. 90＋3. 60） than that in the control group （5.98＋0. 94） （P〈 0. 01） . Meloxicam inhibited apparently the expression levels of COX-2 and PCNA but improved P53 expression in the transplant tumors. Conclusion Meloxicam is effective in inhibiting the growth and inducing apoptosis of hepatocellular carcinoma in vivo.