摘要
The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.
The full length cDNA of a prion protein （PrP） encoding gene of guppy （Poecilia reticulata） and the corresponding genomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame （ORF） of 1545 bp encoding a protein of 515 amino acids, which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length, consisting of 2 introns and 2 exons. The 5＇ untranslated region of cDNA origi- nated from the 2 exons, while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in- cluding brain, eye, liver, intestine, muscle and tail. its transcript was most abundant in the brain. In addition, the transcription of the gene was enhanced by 5 salinity, implying that it was associated with the response of guppy to saline stress.
基金
the Key Laboratory of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences (LFB20070601)
the Key Laboratory of Mariculture of Chinese Ministry of Education,Ocean University of China
National High Technology Research and Development Program of China (2007AA09Z427)
