摘要
BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.
BACKGROUND： Studies have demonstrated that oxyhemoglobin （OxyHb） can induce brain cell apoptosis in vivo.OBJECTIVE： To observe the effects of exogenous nerve growth factor （NGF） on cerebral cortical neuronal Bcl-2 and Bax expression in mice with OxyHb-induced subarachnoid hemorrhage. DESIGN, TIME AND SETTING： A completely randomized grouping, controlled animal experiment was performed at the Experimental Center for Biomedicine, College of Medicine, Xi＇an Jiaotong University between February and April 2005. MATERIALS： Fifty-four healthy, male, adult, ICR mice were included in this study. Subarachnoid hemorrhage was induced by a subarachnoid injection of OxyHb in 48 mice. Mouse NGF was obtained from Xiamen Beidazhilu Bioengineering Co., Ltd., China.METHODS： All 54 mice were randomly divided into three groups： control （n = 6）, injury （n = 24）, and NGF （n = 24）. The NGF group received a subarachnoidal administration of OxyHb, immediately followed by a caudal vein injection of NGF （1 μg）. The injury group was injected with OxyHb, and subsequently with physiological saline. The control group only received intravenous physiological saline. MAIN OUTCOME MEASURES： At 1,6, 24, and 48 hours following subarachnoid hemorrhage induction, expression levels of Bcl-2 and Bax were detected by immunohistochemistry in the cerebral cortex 3 mm anterior and posterior to the injection site. RESULTS： At all time points following OxyHb injection, cerebral cortical Bax levels were significantly higher in the injured group than in the control and NGF groups （P 〈 0.01）. During the first 24 hours following OxyHb injection, cerebral cortical Bcl-2 levels were significantly lower in the injury group compared to the control group （P 〈 0.05 0.01）. Between 1 and 48 hours, Bcl-2 levels were significantly higher in the NGF group than in the injury group （P 〈 0.01 ）. CONCLUSION： Exogenous NGF can inhibit increased neuronal Bax expression and decreased Bcl-2 expression in the cerebral cortex of mice with OxyHb -induced subarachnoid hemorrhage.
