少突胶质细胞发生过程中S-100β表达的变化

Expression changes of S-100β during the oligodendrocyte development

目的:探讨少突胶质细胞发生过程中3个主要不同阶段S-100β的表达。方法:采用振荡法和差速贴壁法,获得纯化的O-2A祖细胞;双重免疫荧光细胞化学显色法观察S-100β在少突胶质细胞发生过程中的表达情况。结果:O-2A祖细胞在含有碱性成纤维细胞因子(10 ng/ml)和血小板源性生长因子(10 ng/ml)培养液中培养48 h,不表达S-100β;换成含有3′碘甲状腺原氨酸(30 ng/ml)培养液继续培养24 h,S-100β免疫反应呈现阳性。前少突胶质细胞、少突胶质细胞中表达S-100β。结论:少突胶质细胞发生过程中S-100β的表达始于O-2A祖细胞过渡到前少突胶质细胞之间,可能与其从多潜能分化阶段过渡到形态学上的分化阶段有关。

Objective： To investigate the expression of S-10013 during the main three stages of the oligodendrocyte development. Methods： 0 2A progenitor cells were purified with a standard shaking method and a differential adhesion method. S- 100β expression were detected in O-2A lineage cells by double immunocytochemistry staining. Results： O-2A progenitor cells were cultured in the presence of platelet-derived growth factor （PI）GF, 10 ng/ml） and basic fibroblast growth factor （bFGF, 10 ng/ml） for 48 hours, which did not express S-100β. When O-2A progenitor cells were shifted to culture medium with triiodothyronine （T3, 30 ng/ml） for 24 hours, they were S-100β positive. Pro-oligodendrocytes and oligodendrocytes were also characterized by S-100β immunoreactivity. Conclusion： The beginning expression of S-100β was between O-2A progenitor and pro oligodendrocyte and may be correlated with the transition from the dividing multipotent stage to the morphological differentiated stage.