电针腧穴“胃病方”对胃黏膜损伤大鼠EGF及EGFR mRNA表达的调节

Effects of electroacupuncture at points "Weibingfang" on EGF and EGFR mRNA expressions in rats with lesion of gastric mucosa

目的：探讨电针腧穴“胃病方”治疗急性胃黏膜损伤的作用机制。方法：将40只清洁级Wistar大鼠随机分为4组,即空白对照组（正常组）、假模型组、模型组、胃病方组,每组10只。采用无水乙醇灌胃法造模。胃病方组取“内关”“中脘”和“足三里”,针刺入穴位后施以疏密波,频率10-30Hz,电流强度2mA,以大鼠肢体微颤为宜,电针20min。治疗结束1h后,取大鼠腹动脉血液及胃黏膜组织,采用酶联免疫吸附法检测血清中表皮生长因子（EGF）及逆转录-多聚酶链反应法测胃黏膜上皮表皮生长因子受体（EGFR）mRNA表达。结果：模型组大鼠血中EGF含量为41.62±12.58ng/L,胃黏膜中EGFR mRNA表达为0.78±0.03,与正常组[（60.37±12.01）ng/L,0.55±0.04]及假模型组[（61.21±13.46）ng/L,0.53±0.05]相比,差异有统计学意义（均P〈0.05）。胃病方组电针后,EGF的含量升高[（70.59±10.14）ng/L],胃黏膜上皮EGFR mRNA表达上调（1.18±0.02）,与模型组相比,差异有统计学意义（均P〈0.05）。结论：腧穴“胃病方”可能促进了胃黏膜上皮细胞增殖、分化、迁移,以修复损伤的胃黏膜。

Objective To probe the mechanism of electroacupuncture at points ＂Weibingfang＂ in treatment of acute gastric mucosal lesion. Methods Forty Wistar rats of sanitary degree were randomly divided into 4 groups, normal group, model group, sham-model group and Weibingfang group, 10 rats in each group. The acute gastric mucosal lesion model was made by intragastric perfusion of anhydrous alcohol. The Weibingfang group were treated by electroacupuncture at ＂Neiguan＂ （PC 6）, ＂Zhongwan＂ （CV 12） and ＂Zusanli＂ （ST 36） with sparse-dense wave, frequency of 10-30 Hz, current intensity of 2 mA, for 20 min. One hour after the treatment, the blood from the abdominal aorta and the gastric mucosa tissue were taken, and serum epidermal growth factor （EGF） level and epidermic growth factor receptor mRNA （EGFR mRNA） expression in the gastric mucosa were detected with enzyme linked immunosorbent assay （ELISA） and reverse transcriptase-polymerase chain reaction （RT-PCR） respectively. Results There were significant differences in blood EGF level （41.62±12.58） ng/L and EGFR mRNA expression （0.78±0.03） in the model group were significantly different from [（60.37±12.01） ng/L and 0.55±0.04] in the normal group and [（61.21±13.46） ng/L and 0.53±0.05] in the sham-model group （P〈0.05）; after electroacupuncture, blood EGF level （70.59±10.14） ng/L increased and the EGFR mRNA expression （1.18±0.02） in the gastric mucosa was up-regulated with a significant differences as compared with those in the model group （P〈0.05）. Conclusion Point ＂Weibingfang＂ can promote proliferation, differentiation and migration of the gastric mucosal epidermic cells to repair the gastric mucosal lesion.