盐裂间接免疫荧光技术在大疱性类天疱疮鉴别诊断中的应用研究

Evaluation on the application of indirect immunofluorescence on salt split skin in differential diagnosis of bullous pemphigoid

目的:探讨盐裂皮肤间接免疫荧光(IIF)技术在大疱性类天疱疮(BP)鉴别诊断中的作用。方法:应用盐裂IIF技术检测78例常规方法诊断为BP的患者血清。结果:43例血清IgG沉积于表皮侧,7例IgG沉积于双侧,11例IgG沉积于真皮侧,另有17例双侧均未见抗体沉积。结论:盐裂IIF仅能用于BP的初步鉴别诊断。

Objective： To evaluate the application of salt split skin indirect immunofluorescence （SSS IIF） in differential diagnosis of bullous pemphigoid （BP）. Methods： Serum samples of 78 patients routinely diagnosed as having BP were tested with SSS IIF. Results： Among the 78 sera tested, IgG was deposited on the side of basic mem- brane zone in 43 sera, on both sides in 7 and on the dermal side in 11. The deposition of IgG were negative in 17 sera. Conclusion： SSS IIF can only be used as an preliminary, differential diagnosis of BP.