尖锐湿疣HPV的分型及宫颈阴道脱落细胞和疣组织中HPV检测的比较

Genotyping of human papillomavirus in patients with condyloma acuminatum and the agreement of HPV genotyping between cervicovaginal smears and matched biopsy specimens

目的:比较阴道脱落细胞和尖锐湿疣(CA)疣组织中HPV的基因型分布和差异。方法:采用液相芯片技术对116例CA的疣组织以及48例女性患者的宫颈阴道脱落细胞进行HPV分型。结果:116例疣组织中有114例HPV阳性,阳性检出率为98.3%,其中主要的基因型是HPV6型(61.4%),HPV11型(42.9%),HPV16型(5.3%),HPV52型(4.39%),HPV18型(3.51%)。宫颈阴道脱落细胞和疣组织标本中HPV亚型的阳性检出率无显著性差异(P>0.05)。结论:上海地区CA患者HPV感染常见的基因型是HPV6、11、16、52和18型。应用宫颈阴道脱落细胞标本可以替代疣组织对HPV感染进行检测。

Objective： To determine the distribution of HPV genotype and to compare the HPV genotyping between eervieovaginal smears and matched biopsy specimens in patients with condyloma aeuminatum （ CA）. Methods： Biopsy samples were obtained from 116 patients with CA. Matched eervicovaginal smears were obtained from 48 fernale patients. Luminex xMAP technology was used to determine the genotype of HPV. Results： Out of 116 biopsy specimens, 114 were positive for HPV （98.3%）. Among the positive specimens 70 were HPV- 6 （61.4%）, 49 HPV- 11 （42.9%）, 6 HPV- 16 （5.3%）, 5 HPV- 52 （4.39%） and 4 HPV- 18 （3.51%）. There was no significant difference in HPV genotyping between matched cervieovaginal smears and biopsy specimens （ P 〉 0.05）. Conclusion： HPV 6, 11, 16, 52, 18 are major genotypes in patients with CA in Shanghai. Cervieovaginal smears can be used to detect the infection of HPV.