摘要
用麻风患者的血清(L38份、B19份、T36份)比较了α1-ELISA、α2--ELISA与PGL-I-ELISA法,结果各型患者血清中的抗体滴度分别为:α1-抗原>α2-抗原>PGL-I,尽管L型的血清中抗α1-抗原的滴度明显高于其它两种抗原,但其阳性率在三种方法之间统计学上没有差别,分别为97%,92%和94%;B和T型血清α1-ELISA法的阳性率明显高于其它两种方法,分别为84%、58%、68%和47%、38%、30%,以α1-ELISA法的敏感性最高。提示α1-ELISA在麻风血清诊断中有很大潜力。
Two techiques of ELISA for the detection of antibodies to M.leprae have been developed. They use the recombinant α1- and α2-antigen of M. leprae,and the sera from leprosy patients (L 38,B 19 and T 36) were analyzed with the techniques.PGL-1 ELISA was used as a reference test. The results showed that the sensitivity of α1- and α2- ELISA and the PGL-1-ELISA on L leprosy sera were 97%,92% and 94% respectively.The corresponding results for B and T leprosy sera were 84%,58%, 68% and 47%, 38%,30% respectively.The α1-ELISA has the highest sensitivity.