摘要
目的:观察"双固一通"针法对甲亢性心肌病大鼠病理组织学的影响。方法:30只SD大鼠,随机分为空白对照组、模型组、"双固一通"组,每组10只。采用腹腔注射L-Thy制备甲亢性心肌病模型,"双固一通"组电针关元、足三里(双)、内关(双)。观察大鼠一般情况(体温、体重、心率等)、病理组织学的变化,放射免疫法检测血清TT3、TT4浓度变化。结果:与空白对照组相比,模型组、"双固一通"组体温明显升高(P<0.05),体重显著降低(P<0.05),心率显著增加(P<0.05);与模型组相比,"双固一通"组心率显著减慢(P<0.05),体温与体重无显著性差异(P>0.05)。"双固一通"组血清TT3和TT4水平明显升高,与模型组相比,有显著性差异(P<0.05)。光镜观察显示模型组心肌纤维肥大,排列紊乱,间隙增宽,可见肌束中断现象;"双固一通"组心肌纤维形态和排列大致正常。结论:"双固一通"针法对甲亢性心肌病大鼠肥厚心肌病理组织学变化具有较明显的改善作用。
Objective: To explore the influence of "Shuanggu Yitong" (Double-reinforcing and One-unblocking) Acupuncture on the pathologic histology in rats with hyperthyroid cardiomyopathy. Methods:30 SD rats were equally randomized into 3 groups as: control group,model group, Shuanggu Yitong group. The hyperthyroid cardiomyopathy was induced by an intraperitoneal injection of L-Thy(L-thyroxine)in the SD rats. Electro-Acupuncture at Guanyuan(CV4),bilateral Zusanli (ST 36)and bilateral Neiguan (PC 6)was applied to the rats in Shuanggu Yitong group. The rats were observed generally (temperature,weight,heatbeat and pathological histology). The concentrations of TI'3 and TI'4 in blood serum were measured by radioimmunoassay. Results:Compared with the control group,the body temperature of model group and "Shuanggu Yitong"group increased obviously (P〈0.05),the weight reduced obviously (P〈0.05),the heart rate raised obviously (P〈 0.05). Compared with the model group,the heart rate of Shuanggu Yitong group was lower than that of model group (P〈 0.05) ,body temperature and weight were not different significantly (P〉0.05). Compared with the model group,the serum TT3 of Shuanggn Yitong group and TT4 levels went up obviously (P〈0.05). Through light microscope,myocardial fiber in model group the was hypertrophy, arrange mess, the cleft increases. It was clear that the muscle bundle interrupted. Appearance and alignment of the myocardial fiber of "Shuanggu Yitong"group were mostly normal. Conclusion : Shuanggu Yitong therapy improves thick myocardial pathologic histology changes of hyperthyroid cardiomyopathy rats.
出处
《世界中西医结合杂志》
2008年第6期319-321,共3页
World Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局基金资助项目(No02-03JP28)
关键词
甲状腺机能亢进症
心肌肥厚
电针/“双固一通”针法
病理组织学
大鼠
Hyperthyroid cardiomyopathy
cardiac hypertrophy
electro -acupuncture Shuanggu Yitong acupuncture
pathologic histology
rats