肾衰养真胶囊对慢性肾功能衰竭腹膜透析营养不良大鼠Leptin及OB mRNA表达的影响

Effect of Shenshuai Yangzhen Jiaonang on Expressions of Leptin and OB mRNA in CRF-PD Malnourished Rats

目的:研究肾衰养真胶囊对慢性肾功能衰竭(CRF)腹膜透析(PD)营养不良大鼠血浆瘦素(Leptin)及OB mR-NA表达的影响。方法:SD大鼠40只,雌雄各半,0.5%腺嘌呤饲料喂养10 w建立CRF大鼠模型;再植入腹膜透析管,进行PD 2 w,建立CRF营养不良大鼠模型。造模成功后,随机分为3组:肾衰养真胶囊组、肾灵组和模型对照组,另设正常对照组。肾衰养真胶囊组与肾灵组分别给予相应药物混悬液灌胃,对照组灌服等量生理盐水,治疗4 w后,检测血浆Leptin水平,用RT-PCR方法检测编码Leptin的基因-OB基因mRNA表达情况。结果:造模后大鼠血清中Leptin水平和OB mRNA显著升高,与模型组比较,肾衰养真胶囊组和肾灵组治疗后能显著降低上述指标(P<0.01),且肾衰养真胶囊组治疗效果优于肾灵组(P<0.01)。结论:肾衰养真胶囊能减轻CRF营养不良大鼠血浆Leptin蓄积,下调OB基因mRNA的表达。

Objective：To study the effect of Shenshuai Yangzhen Jiaonang （Jiaonang）on the expressions of leptin and OBmRNA in rats with CRF-PD（chronic renal failureperitoneal dialysis）and malnutrition. Methods：40 SD rats of either sex were fed with the forage with 0.5% adenine contained for lOw for the establishment of CRF models. Dialytic pipe was implanted and dialysis was underway for 2w, thus, CRF malnutrition rats were modeled. After successful modeling, 3 groups were randomized,namely Jiaonang group,Shen Ling group and model control group. Additionally,normal control group（ 10 rats）was set up. Suspension irrigation was applied with corresponding drug administrated in each medicinal group and e- qual-dose physical saline was irrigated in control group. 4w later after treatment,blood leptin level was tested. RT-PCR method was used to determine code leptin gene and OBmRNA expression. Results：After modeling, serum leptin and OBmRNA were up-leveled significantly in rats. Comparing with model group, after treated with Shenshuai Yangzhen Jiaonang and Shen Ling, the above indexes were reduced significantly （P〈0.01）and the effect of Shenshuai Yangzhen Jiaonang was superior to that of Shen Ling （P〈0.01）. Conclusion ： Shenshuai Yangzhen Jiaonang alleviates plasma leptin accumulation and down-regulates OBmRNA expression in CRF malnutrition rats.