阿托伐他汀对华法林致大鼠主动脉中膜钙化及收缩压升高的影响

Effect of atorvastatin on aortic medial calcification and systolic blood pressure of rats induced by warfarin

目的:探讨阿托伐他汀对华法林所致大鼠主动脉中膜钙化和收缩压的影响。方法:将健康成年Wistar大鼠30只随机分为华法林组(10只)、阿托伐他汀组(10只)和正常对照组(10只)。华法林组连续用华法林灌胃4周,正常对照组连续用等量生理盐水灌胃4周,他汀组在用华法林灌胃(15mg.kg-1.d-1)的同时给予阿托伐他汀(20mg.kg-1.d-1)灌胃。每周测鼠尾动脉压1次。4周后,取大鼠主动脉。观察弹性纤维和胶原纤维,以及中膜钙盐沉积。结果:华法林组动脉中膜有钙盐沉积,而他汀组钙盐沉积较华法林组明显减少。华法林组收缩压明显增高,而他汀组则明显降低由华法林诱导的大鼠收缩压升高(P<0.01)。结论:华法林能使大鼠主动脉中膜钙盐沉积,从而升高其收缩压;而阿托伐他汀能抑制华法林的上述作用,提示阿托伐他汀可能通过抑制华法林所致动脉中膜钙化而降低其收缩压。

OBJECTIVE To understand the effect of atorvastatin to aortic medial calcification and systolic blood pressure （SBP） of rats induced by warfarin. METttODS Healthy and adultrats were randomly divided into warfarin group （ 15 mg·kg^-1·d^-1 intragastric administration）, atorvastatin group （20 mg·kg^-1·d^-1 intragastric administration） and normal control group. Caudal arterial pressure of rats were measured once a week, then got aorta after 4 weeks. Elastic fiber, collagen fiber and calcium accumulation in tunica media of aorta were measured by Von Kossa staining. RESULTS The warfarin treatment led to elevation of systolic blood pressure and aortic medial calcification. This chronic treatment also increased collagen, whereas elastin decreased in the aorta. However, the atorvastatin treatment had adverse effects. CONCLUSION Treatment with atorvastatin represent evidence of BP lowing and calcification reducing. These data demonstrate that atorvastatin protects aortic medial from warfarin-induced calcification and elevation of systolic blood pressure.