甘草总黄酮微丸溶出度的考察

Determination of the dissolution of glycyrrhiza total flavones pellets by ultraviolet spectrophotometry

目的:建立甘草总黄酮微丸溶出度的测定方法。方法:依据中国药典2005年版二部附录XC,采用桨法测定,以1.0%十二烷基硫酸钠的pH6.8磷酸盐缓冲溶液为溶出介质,转速为100r.min-1,紫外分光光度法检测。结果:以甘草苷为对照品计算累积溶出率,45min时均大于75%,在4.07～24.42μg的范围内,浓度与其吸收度呈良好线性关系,平均回收率为100.61%,RSD为0.95%。结论:经过溶出条件的系统筛选后所建立的方法稳定,结果可靠。

OBJECTIVE To establish a method for determining the dissolution of glycyrrhiza total flavones pellets by Ultraviolet spectrophotometry. METHODS The determination was performed at. the detection wavelength 05 335nm, solvent： 1.0%SDS-pH 6. 8 phosphate buffer, paddle rate： 100 r·min^-1, temperature： 37 ℃. RESULTS Compared with liquiritin as a standard, the cumulative dissolution percentage exceeded 75% in 45 minutes. Glycyrrhiza total flavones concentration presented a good linear range of 4. 07-24. 42μg; the average recovery was 100. 61% with RSD of 0. 95%. CONCLUSION The method is simple, accurate and reliable for determining the dissolution of glycyrrhiza total flavones.