关于脂溶性化感物质研究方法的商榷

Discussion of Research Methods for Fat-soluble Allelochemicals

在有关化感物质的研究中,由于一些观念和实验方法的限制,对于脂溶性物质在自然生态环境中是否有化感作用存在争议。在化感物质的生物检测中,脂溶性成分的实验方法比较混乱,并存在很多问题,直接影响到实验的准确性和有效性,不能客观科学地进行定性及定量测定。植物的化感作用是在一个复杂的生态体系中发生的,不应主观排除脂溶性物质在自然界中的化感作用,尤其是现在化感作用机理研究越来越成为研究的热点和重点,要阐明化感作用的分子化学、酶调控和基因调节机制,迫切需要建立一个目标植物全成分化感活性筛选的基础平台,并建立定量化感活性生物检测方法。

In allelocchemicals researches, the fat-soluble components causing allelopathy in natural ecological environment or not is still controversial as a result of limited concepts and experimental methods. Actually, the current bioassay methods of fat-soluble components have lots of disadvantages, which directly affect the validity and accuracy of allelopathieal experiments. Plant allelopathy occurs in the complex ecosystem, we can not ignore the allelopathy of fat-soluble substances subjectively along with allelopathy mechanism is attracting the attention of more and more researchers. Therefore, it is urgent to establish a platform of wide components selection of allelopathy activity to elucidate the allelopathy mechanism of the molecular chemistry, enzyme and gene regulation.