摘要
本文用反相高效液相色谱法测定垫状卷柏药材中的穗花杉双黄酮。选用Purospher star RP-C18色谱柱（250mm×4．6mm，5μm），以甲醇-0．1％磷酸水溶液（65：35）为流动相，流速为O．8mL／min，检测波长337nm，柱温25℃进行检测。结果显示，穗花杉双黄酮的含量在0．038—0．342μg范围内时进样量与峰面积积分值呈良好的线性关系，r=0．9999，平均回收率为101．2％，RSD为2．3％（n=6）。该方法测定垫状卷柏药材中的穗花杉双黄酮含量灵敏、准确、重现性好。
In this paper we established a RP-HPLC method for the amentoflavone in Selaginella pulvinate and determined the quantity of amentoflavone. The mobile phase of chromatographic conditions,including Purospher star RP-C18 column （250 mm ×4.6 ram, 5 pan）, is consisted of methanol-0. 1% phosphoric acid solution （ 65： 35 ）. The detection wavelength was at 337 nm and the temperature of the column was 25 ℃. Amentoflavone showed a good linear relationship at the range of 0.038-0.342 μg,r =0. 9999. The recovery rate was 101.2% and RSD was 2.3%. The methods can be used accurately and quickly for the quality control of amentoflavone in Selaginella pulvinate.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第5期863-865,共3页
Natural Product Research and Development
基金
国家自然科学基金(30500646)
河南省卫生厅艾滋病科技攻关计划(2006038)
