摘要
本文对八角茴香中莽草酸的提取和纯化工艺进行优化研究。结果表明,莽草酸的最佳提取工艺条件为:常规水浸提,温度80℃,固液比1∶15,提取时间100min,莽草酸的提取量达到0.1795g。最佳纯化工艺:莽草酸浸膏搅拌硅胶,柱层析,乙酸乙酯洗脱,莽草酸洗脱量达到0.1742g,真空浓缩至干,甲醇和水混合结晶,得含量98%的纯品莽草酸。
Conditions of the extraction and purification of shikimic acid in lllicium verum Hook. f. were optimized by orthogonal experiment in this paper. The results showed as follows ： traditional extraction; the optimum conditions for the extraction process is 80 ℃ ,water ratio 1：15 and time 100 min;the extraction yield of shikimic acid could achieve 0. 1795 g. The optimum conditions for purification process is the shikimic acid extract mixing silicone, column chromatography, ethyl acetate elution;the yield of shikimic acid could achieve 0. 1742 g; Vacuum concentrate drying; crystallization in the mixture of methanol and water,the shikimic acid content is 98%.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第5期914-917,共4页
Natural Product Research and Development
基金
国家863计划海洋技术领域重点项目(2007AA091702)
国家支撑计划项目
关键词
水提取
八角茴香
柱层析
结晶
莽草酸
traditional extraction
lllicium verum Hook. F.
column chromatography
crystallization
shikimic acid
