期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

热休克蛋白47重组质粒对糖尿病大鼠皮肤创口愈合影响的实验研究 被引量：1

Effects of HSP47 Recombinant Plasmid on the Skin Wound Healing Process in the Alloxan-induced Diabetic Rats
下载PDF
导出
摘要 目的:初步研究热休克蛋白47(HSP47)重组质粒pTracer-CMV-HSP47局部注射对糖尿病大鼠皮肤创口愈合的影响,探讨以HSP47质粒为基础的基因治疗促进糖尿病皮肤创口愈合的可行性和有效性。方法:制备四氧嘧啶诱导的糖尿病大鼠皮肤创口愈合模型,创缘皮下局部注射HSP47重组质粒,观察其对皮肤创口愈合的影响,并用免疫组织化学、荧光定量RT-PCR、Western blotting检测创口愈合过程中HSP47和collagen I的表达变化。结果:在糖尿病大鼠皮肤创缘连续两次注射质粒后第3、6、9天,免疫组织化学、荧光定量RT-PCR和Western blotting检测证实,HSP47重组质粒组比对照组能显著增加创口中的HSP47和collagenⅠ的表达。结论:在糖尿病大鼠皮肤创口周围注射质粒pTracer-CMV-HSP47,能明显增强HSP47和collagenⅠ的表达,有助于改善创口愈合,HSP47可能是促进糖尿病皮肤创口愈合的潜在治疗靶点。 Objective： To investigate the effects of local injection of HSP47 recombinant plasmid on the skin wound healing process in alloxan-indueed diabetic rats and assess the possibility and utility of gene therapy based on HSP47 plasmid. Methods： Diabetic rats with skin wound were randomly distributed into 3 groups. Group A wounds injection normal saline, group B injection pTracer-CMV, group C injection pTraeer-CMV-HSP47. Immunohistochemistry. quantitative fluorescent RT-PCR, Western blotting techniques during 3, 6. 9 d after plasmid injection were used to evaluate tile expression changes of HSP47 and collagen Ⅰ. Results： After two consecutive local plasmid injections, the expression of HSP47 and collagen Ⅰ in pTracer-CMV-HSP47 group was concomitantly strengthened and much intensify than those in A and B group. Conclusion： The expression of HSP47 and collagen Ⅰ were considerahly enhanced after pTraeer-CMV- HSP47 local injection, which may help to improve the wound healing in the diabetic rats. The data suggests that HSP47 may possibly become a potential therapeutic target to promote diabetic wound healing in future.
作者 程杰 李琳 王佐林
机构地区 上海交通大学医学院附属第九人民医院口腔颌面外科 同济大学附属口腔医院口腔颌面外科
出处 《口腔颌面外科杂志》 CAS 2008年第5期309-313,共5页 Journal of Oral and Maxillofacial Surgery
关键词 热休克蛋白47 糖尿病鼠 皮肤创口愈合 基因治疗 47kD heat shock protein- diabetic rat skin wound healing gene therapy
分类号 R587.1 [医药卫生—内分泌] R641 [医药卫生—外科学]
  • 相关文献

参考文献19

  • 1Falanga V. Wound healing and its impairment in the diabetic foot [J]. Lancet, 2005, 366(9498):1736-1743.
  • 2Brown DL, Kane CD. Chemausek SD, et al. Differential expression and localization of insulin-like growth factors 1 and II in cutaneous wounds of diabetic and nondiabetie mice [J]. Am J Pathol,1997, 151(3):715-724.
  • 3Beer HD, Longaker MT, Wemer S, et al. Reduced expression of PDGF and PDGF receptors during impaired wound healing [J]. J Invest Dermatol, 1997. 109(2): 132-138.
  • 4Schaffer MR, Tantiy U, Efron PA. et al. Diabetes-impaired healing and reduced wound nitric oxide synthesis: a possible pathophysiologic correlation [J]. Surgery.1997.121(5): 513-519.
  • 5Bitar MS. Labbad ZN. Transforming growth factor-beta and insulin-like growth factor-I in relation to diabetes-induced impairment of wound healing [J]. J Surg Res. 1996. 61(1): 113-119.
  • 6Nagata K. HSP47 as a collagen-specific molecular chaperone: function and expression in normal mouse development [J]. Semin Cell Dev Biol, 2003, 14(5):275-282.
  • 7Rocnik EF, van der Veer E, Cao H, et al. Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells [J]. J Biol Chem, 2002,277(41):38571-28578.
  • 8Wang ZL, Inokuchi T. Nemoto TK, et al. Antisense oligonucleotide against collagen-specific molecular chap- erone 47-kDa heat shock protein suppresses scar formation in rat wounds [J]. Plast Reconstr Surg, 2003;111 (6): 1980-1987.
  • 9Keagle JN, Welch W J, Young DM. Expression of heat shock proteins in a linear rodent wound[J]. Wound Repair Regen, 2001,9(5):378-385.
  • 10Razzaque MS, Taguehi T. The possible role of colligin/ HSP47, a collagen-binding protein, in the pathogenesis of human and experimental fibrotic diseases [J]. Histol Histopathol, 1999,14(4): 1199-1212.

二级参考文献30

  • 1王佐林,邱蔚六,井口次夫,根本孝幸.HSP47在胎鼠皮肤创口愈合中的表达[J].口腔颌面外科杂志,2004,14(2):95-99. 被引量：2
  • 2王佐林,张萍,陈万涛,张志愿,邱蔚六.HSP47和TGFβ1在大鼠皮肤瘢痕形成中的作用[J].口腔颌面外科杂志,2004,14(4):293-298. 被引量：5
  • 3程杰,王佐林.大鼠热休克蛋白47真核表达载体的体外构建[J].口腔颌面外科杂志,2007,17(1):6-10. 被引量：2
  • 4Dafforn TR,Della M,Miller AD.The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis[J].J Biol Chem,2001,276(52):49310-49319.
  • 5Koide T,Asada S,Takahara Y,et al.Specific recognition of the collagen triple helix by chaperone HSP47:minimal structural requirement and spatial molecular orientation[J].J Biol Chem,2006,281(6):3432-3428.
  • 6Koide T,Nishikawa Y,Asada S,et al.Specific recognition of the collagen triple helix by chaperone HSP47.Ⅱ.The HSP47-binding structural motif in collagens and related proteins[J].J Biol Chem,2006,281(16):11177-11185.
  • 7Wang ZL,Inokuchi T,Ikeda H,et al.Collagen-binding heat shock protein HSP47 expression during healing of fetal skin wounds[J].Int J Oral Maxillofac Surg,2002,31(2):179-184.
  • 8Razzaque MS,Taguchi T.The possible role of colligin/HSP47,a collagen-binding protein,in the pathogenesis of human and experimental fibrotic diseases[J].Histol Histopathol,1999,14(4):1199-1212.
  • 9Kakugawa T,Mukae H,Hayashi T,et al.Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia[J].Respir Res,2005,6(1):57-60.
  • 10Ohashi S,Abe H,Takahashi T,et al.Advanced glycation end products increase collagen-specific chaperone protein in mouse diabetic nephropathy[J].J Biol Chem,2004,279(19):19816-19823.

共引文献1

同被引文献11

  • 1L(o)e H. Periodontal disease.The sixth complication of diabetes mellitus[J].{H}DIABETES CARE,1993,(01):329-334.
  • 2Zhen D,Chen Y,Tang X. Metformin reverses the deleterious effects of high glucose on osteoblast function[J].{H}Journal of Diabetes and its Complications,2010,(05):334-344.
  • 3Gopalakrishnan V,Vignesh RC,Arunakaran J. Effects of glucose and its modulation by insulin and estradiol on BMSC differentiation into osteoblastic lineages[J].{H}Biochemistry and Cell Biology-Biochimie et Biologie Cellulaire,2006,(01):93-100.
  • 4Jin P,Zhang X,Wu Y. Streptozotocin-induced diabetic rat-derived bone marrow mesenchymal stem cells have impaired abilities in proliferation,paracrine,antiapoptosis and myogenic differentiation[J].{H}TRANSPLANTATION PROCEEDINGS,2010,(07):2745-2752.
  • 5Colombo JS,Balani D,Sloan AJ. Delayed osteoblast differentiation and altered inflammatory response around implants placed in incisor sockets of type 2 diabetic rats[J].{H}Clinical Oral Implants Research,2011,(06):578-586.
  • 6Botolin S,Faugere MC,Malluche H. Increased bone adiposity and peroxisomal proliferator -activated receptor-gamma 2 expression in type I diabetic mice[J].{H}ENDOCRINOLOGY,2005,(08):3622-3631.
  • 7Kim HS,Park JW,Yeo SI. Effects of high glucose on cellular activity of periodontal ligament cells in vitro[J].{H}Diabetes Research and Clinical Practice,2006,(01):41-47.
  • 8Li YM,Schilling T,Benisch P. Effects of high glucose on mesenchymal stem cell proliferation and differentiation[J].{H}Biochemical and Biophysical Research Communications,2007,(01):209-215.
  • 9Yu M,Zhou W,Song Y. Development of mesenchymal stem cell-implant complexes by cultured cells sheet enhances osseointegration in type 2 diabetic rat model[J].{H}BONE,2011,(03):387-394.
  • 10Wikesj(o) UM,Sorensen RG,Wozney JM. Augmentation of alveolar bone and dental implant osseointegration:clinical implications of studies with rhBMP-2[J].{H}Journal of Bone and Joint Surgery-American Volume,2001,(Pt 2):S136-S145.

引证文献1

二级引证文献2

口腔颌面外科杂志
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部